TrueCut™ Cas9 Protein v2
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TrueCut™ Cas9 Protein v2
Invitrogen™

TrueCut™ Cas9 Protein v2

A next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency.
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Catalog NumberQuantityConcentration
A3649725 μg1 μg/μL
A36498100 μg5 μg/μL
A3649610 μg1 μg/μL
A36499500 μg5 μg/μL
Catalog number A36497
Price (USD)/ Each
112.65
Online Exclusive
116.00
Save 3.35 (3%)
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Quantity:
25 μg
Concentration:
1 μg/μL
Price (USD)/ Each
112.65
Online Exclusive
116.00
Save 3.35 (3%)
Add to cart
Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency.

Features include:

  • Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2X higher editing efficiency in difficult targets than the competition
  • High quality—manufactured under strict ISO 13485 quality standards
  • Validated protocols for a large number of cell types help you achieve success faster

TrueCut Cas9 Protein v2 is recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aids delivery to the nucleus, increasing the rate of genomic DNA cleavage.

  • Transfection-ready, using either lipid-mediated transfection reagents or electroporation
  • Eliminates time-consuming cloning steps
  • Available in 2 concentrations:
    • 1 μg/μL for use in standard editing scenarios
    • 5 μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
    • For custom sizes or concentrations, please inquire

Options for obtaining CRISPR gRNA for use with TrueCut Cas9 Protein v2:

  • Order transfection-ready TrueGuide synthetic gRNAs
  • Generate transfection-ready gRNA in as little as four hours including template assembly with our GeneArt Precision gRNA Synthesis Kit (Cat. No. A29377)
  • Have our Custom Services team design, synthesize, and purify in vitro (IVT) gRNA sequences for you
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Accession NumberNC_002737.2:854751-858857
ComponentsTrueCut Cas9 Protein v2
Expression SystemE. coli
Gene Aliascas5, csn1
Gene ID (Entrez)901176
Gene SymbolSpy_1046
GradeMolecular Biology
Product TypeCas9 Protein v2
Protein FormRecombinant
Protein TagUntagged
Quantity25 μg
Shipping ConditionDry Ice
Validated ApplicationGenome Editing
Concentration1 μg/μL
Product LineTrueCut™
Research CategoryGenome Editing
Unit SizeEach
Contents & Storage
TrueCut Cas9 Protein v2 (1 mg/mL), 25 μg

Store at -5°C to -30°C.