TrueCut™ Cas9 Protein v2
TrueCut™ Cas9 Protein v2
인용 및 참조 문헌 (3)
Invitrogen™

TrueCut™ Cas9 Protein v2

A next-generation CRISPR Cas9 protein engineered to deliver maximum on-target editing efficiency. TrueCut Cas9 Protein v2 is ideal for applications requiring the highest editing efficiency.
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호수량농도
A3649725 μg1 μg/μL
A36498100 μg5 μg/μL
A3649610 μg1 μg/μL
A36499500 μg5 μg/μL
카탈로그 번호 A36497
제품 가격(KRW)
209,000
온라인 행사
Ends: 31-Dec-2025
219,000
할인액 10,000 (5%)
Each
카트에 추가하기
수량:
25 μg
농도:
1 μg/μL
제품 가격(KRW)
209,000
온라인 행사
Ends: 31-Dec-2025
219,000
할인액 10,000 (5%)
Each
카트에 추가하기

Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency.

Features include:

  • Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2X higher editing efficiency in difficult targets than the competition
  • High quality—manufactured under strict ISO 13485 quality standards
  • Validated protocols for a large number of cell types help you achieve success faster

TrueCut Cas9 Protein v2 is recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aids delivery to the nucleus, increasing the rate of genomic DNA cleavage.

  • Transfection-ready, using either lipid-mediated transfection reagents or electroporation
  • Eliminates time-consuming cloning steps
  • Available in two concentrations:
    • 1 μg/μL for use in standard editing scenarios
    • 5 μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
    • For custom sizes or concentrations, please inquire

Options for obtaining CRISPR gRNA for use with TrueCut Cas9 Protein v2:

  • Order pre-designed TrueGuide Synthetic gRNAs for high-efficiency knock out in human or mouse
  • Submit your own design for custom synthesis of CRISPR sgRNA
  • Use our free TrueDesign CRISPR Genome Editor software for easy design and ordering of custom gRNA for precise genome editing
For Research Use Only. Not for use in diagnostic procedures.
사양
등록 번호NC_002737.2:854751-858857
구성 요소TrueCut Cas9 Protein v2
발현 시스템E. coli
유전자 별칭cas5, csn1
유전자 ID(Entrez)901176
유전자 기호Spy_1046
등급Molecular Biology
제품 유형Cas9 Protein v2
단백질 형태Recombinant
단백질 태그Untagged
수량25 μg
배송 조건Dry Ice
검증된 애플리케이션Genome Editing
농도1 μg/μL
제품라인TrueCut
연구 카테고리Genome Editing
Unit SizeEach
구성 및 보관
TrueCut Cas9 Protein v2 (1 mg/mL), 25 μg

Store at -5°C to -30°C.

자주 묻는 질문(FAQ)

What is the molecular weight of TrueCut Cas9 Protein v2?

The molecular weight of TrueCut Cas9 Protein v2 is ~163 kD

What is the difference between TrueCut Cas9 Protein (Prototype) and TrueCutCas9 Protein v2?

Both products are the same CRISPR-Cas9 protein, but the TrueCut Cas9 Protein is manufactured with a new production process and formulated to meet the standards for Ancillary Materials for Cell and Tissue-Based Products.

Is there a specific antibody that you recommend to test the delivery of TrueCut Cas9 Protein v2?

Although we do not have a specific antibody recommendation to test the delivery of TrueCut Cas9 Protein v2, we have tested the following antibodies against an older version of Cas9 (Platinum Cas9) using U2OS-Cas9 expression cell lines (generated using Cas9 lentivirus):
- Cas9 Recombinant Polyclonal Antibody (Cat. No. 711061)
- Cas9 Monoclonal Antibody (Cat.No. MA1-201)
- Cas9 Monoclonal Antibody (Cat. No. MA1-202)

Our TrueCut Cas9 Protein v2 and the Platinum Cas9 have similar domains, and the TrueCut Cas9 Protein v2 has better efficiencies. Therefore, these antibodies should work with TrueCut Cas9 Protein v2 as well.

What is the storage buffer composition for TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

The storage buffer composition for TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein is as follows:
15 mM Tris-HCl pH 8.0, 150 mM NaCl, 1.0 mM TCEP, 50% glycerol.

Which lipid transfection reagent do you recommend using with TrueCut Cas9 Protein v2 and TrueCut HiFi Cas9 Protein?

We recommend using Lipofectamine CRISPRMAX Transfection Reagent with TrueCut Cas9 Protein v2 or TrueCut HiFi Cas9 Protein.

View all TrueCut™ Cas9 Protein v2 FAQs

인용 및 참조 문헌 (3)

인용 및 참조 문헌
Abstract
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins.
Authors:Kim S, Kim D, Cho SW, Kim J, Kim JS
Journal:
PubMed ID:24696461
'RNA-guided engineered nucleases (RGENs) derived from the prokaryotic adaptive immune system known as CRISPR (clustered, regularly interspaced, short palindromic repeat)/Cas (CRISPR-associated) enable genome editing in human cell lines, animals, and plants, but are limited by off-target effects and unwanted integration of DNA segments derived from plasmids encoding Cas9 and guide ... More
Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection.
Authors:Liang X, Potter J, Kumar S, Zou Y, Quintanilla R, Sridharan M, Carte J, Chen W, Roark N, Ranganathan S, Ravinder N, Chesnut JD,
Journal:
PubMed ID:26003884
'CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian cell engineering. However, the delivery of Cas9 and synthesis of guide RNA (gRNA) remain as steps that can limit overall efficiency and ease of use. Here we describe methods for rapid synthesis of ... More
Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA.
Authors:Liang X, Potter J, Kumar S, Ravinder N, Chesnut JD
Journal:J Biotechnol
PubMed ID:27845164
'While CRISPR-based gene knock out in mammalian cells has proven to be very efficient, precise insertion of genetic elements via the cellular homology directed repair (HDR) pathway remains a rate-limiting step to seamless genome editing. Under the conditions described here, we achieved up to 56% targeted integration efficiency with up ... More