Ion AmpliSeq™ HD Library Kit - FAQs

10X DNA working panel FWD and REV subpools can be stored at 4 degrees C for one week. For longer term storage, aliquot and store subpools at -20 degrees C.

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There have been some recent changes to the protocol. Please refer to the latest user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017392_Ion%20AmpliSeqHDLibraryKit_UG.pdf) for full details.

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CRC is a component of the Ion AmpliSeq HD Library Kit (Cat. No. A37694). CRC improves target amplification reactions for challenging panels that produce excessive amounts of primer dimer or panels that have ⋝500 primer pairs. We recommend using CRC with all panel types. HD Enhancer is a component of the Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) and is also available separately as Cat. No. A53690. HD Enhancer is used for improved library quality and molecular coverage results. Guidelines for using HD Enhancer or CRC for Ion AmpliSeq HD library preparation are outlined in the user guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017392_Ion%20AmpliSeqHDLibraryKit_UG.pdf).

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We recommend using the Ion AmpliSeq HD Dual Barcode Kit 1-24 (Cat. No. A37695) with Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283). The libraries are dual barcoded at the 5' and 3' ends and this enables the multiplexing of up to 24 samples in a single chip. Using these dual barcodes also reduces the risk of barcode cross-contamination.

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Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) is shipped on dry ice and we recommend storing it at -20 degrees C.

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Yes, the HD Enhancer, Cat. No. A53690 can be ordered separately by contacting Customer Service.

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The HD Enhancer improves library quality and molecular coverage results. It also improves performance by reducing primer dimers and improves amplicon performance especially GC amplicon molecular coverage.

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Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) has 2 subcomponents - Ion AmpliSeq HD Library Kit (Cat. No. A37694) and HD Enhancer (Cat. No. A53690).

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Ion AmpliSeq HD Library Kit with HD Enhancer (Cat. No. A57283) is sufficient to run 24 reactions.<

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No, it is only compatible with Ion AmpliSeq HD panels.

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The Ion AmpliSeq HD Library Kit, when combined with Ion AmpliSeq HD panels, enables construction of ultra-high sensitivity panels with a limit of detection (LOD) as low as 0.1%.

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