Genexus™ Sequencing Kit
Genexus™ Sequencing Kit
Ion Torrent™

Genexus™ Sequencing Kit

The Ion Torrent Genexus Sequencing Kit provides the materials required to perform two initializations and automated sequencing of two multilaneRead more
Have Questions?
Catalog NumberQuantity
A402712 Runs
Catalog number A40271
Price (HKD)
29,035.00
Each
Add to cart
Quantity:
2 Runs
Price (HKD)
29,035.00
Each
Add to cart
The Ion Torrent Genexus Sequencing Kit provides the materials required to perform two initializations and automated sequencing of two multilane chips using the Ion Torrent Genexus Integrated Sequencer. These innovative sequencing consumables are stable on the instrument for up to two weeks after initialization, allowing flexibility to use the chip lanes according to sample throughput needs. Genexus Bottles 1, 2, and 3 are shelf-stable and the Genexus Cartridge has a minimized footprint, limiting the required storage space at -20°C.

As part of the Genexus Integrated Sequencer workflow, the Genexus Sequencing Kit:
• Automates sequencing for 200–400 base-read Ion AmpliSeq or Ion AmpliSeq HD libraries
• Supports intake variability of clinical research samples in a cost-effective manner
• Maximizes flexibility with the ability to sequence one to four chip lanes per run
• Enables parallel processing of up to four compatible assays in a single run
• Delivers fast and simple instrument setup, with less than five minutes of hands-on time

The Ion Torrent Genexus Integrated Sequencer automates library preparation, sequencing, analysis, and reporting in a hands-off, walkaway workflow to deliver results in a single day. The innovative, multilane sequencing chip is designed to enable cost-effective processing of 1 to 32 single pool libraries.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Comparative Sequencing (Somatic Mutations - 10:50)
For Use With (Equipment)Genexus™ Integrated Sequencer
Product LineGenexus
Product TypeSequencing Kit
Quantity2 Runs
Shipping ConditionQualified for Ambient or Wet Ice
FormatKit
Unit SizeEach
Contents & Storage
• Genexus Cartridge, store at -10 to -30°C
• Genexus Bottle 2, store at room temperature
• Genexus Bottles 1 and 3, store at room temperature

Frequently asked questions (FAQs)

What are the correct shipping conditions for the Genexus Cartridges that are a part of the Genexus Sequencing Kit (Cat. No. A40271) and Genexus GX5 Starter Packs (Cat. Nos. A40279, A40280)?

The Genexus Cartridge should be shipped on supergels at -15 to -50 degrees C, in a polystyrene box. If the cartridges are shipped with dry ice or arrive thawed, they should not be used, and replacement would need to be issued.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the level of detection for somatic variants with the Oncomine Myeloid Research Assay GX?

Detection of somatic variants with the Oncomine Myeloid Research Assay GX has been verified down to 5% allele frequency.

Which extraction kits are compatible with Oncomine Myeloid Research Assay GX?

Here is a list of kits that are compatible with Oncomine Myeloid Research Assay GX:

Nucleic acid isolation - RNA samples
- MagMAX mirVana Total RNA Isolation Kit, Cat. No. A27828
- PureLink Total RNA Blood Kit, Cat. No. K156001
- RNaseZap RNase Decontamination Solution, Cat. No. AM9780, AM9782, AM9784

Nucleic acid isolation - DNA samples
- MagMAX DNA Multi-Sample Ultra 2.0 Kit, Cat. No. A36570
- PureLink Genomic DNA Mini Kit, Cat. No. K1820-01

What are the sample input requirements for Oncomine Myeloid Research Assay GX?

The recommended minimum sample input is 27.75 ng of DNA (1.11 ng/µL minimum concentration) and 14.25 ng of RNA (0.95 ng/µL minimum concentration).

What sample controls should I use with the Oncomine Myeloid Research Assay GX?

The recommended controls are:

DNA Controls:
- Seraseq® Myeloid Mutation DNA Mix, Cat. No. 0710-0408 (SeraCare®)
- Myeloid DNA Reference Standard, Cat. No. HD829 (Horizon™)
- AcroMetrix™ Oncology Hotspot Control, Cat. No. 969056

RNA Controls:
- Universal Human Reference RNA, Cat. No. 740000 (Agilent™)
- Seraseq® Myeloid Fusion RNA Mix, Cat. No. 0710-0407 (SeraCare®)