Gibco™

Expi293™ Met (–) Protein Labeling Kit

Catalog number:  A41249

Related applications:

Mammalian Cell Culture

Back to top

Description

The Expi293 Met (–) Protein Labeling Kit includes Expi293 Met (–) Expression Medium and the ExpiFectamine 293 Met (–) Transfection Kit for rapid, high-yield transient protein expression from Expi293F cells for applications where labeling of methionine residues in proteins is desired. The kit components are specifically formulated without methionine, allowing users to add labeled methionine of their choice.

Expi293 Met (–) Protein Labeling Kit features include:
• Methionine-free medium and transfection enhancer formulations
• 2- to 10-fold higher protein yields compared to other transfection reagents used for high-density 293 cell culture
• Same transient transfection protocol as ExpiFectamine 293 Transfection Kit
• Scalable transfection from small to large scale while maintaining equivalent volumetric protein yields

The Expi293 Met (–) Protein Labeling Kit is specifically designed to easily integrate with other components of the Expi293 Expression System, including Expi293F cells and Expi293 Expression Medium. The components included in the Expi293 Met (–) Protein Labeling Kit are: 1 L Expi293 Met (–) Expression Medium and one ExpiFectamine 293 Met (–) Transfection Kit sufficient to transfect 1 L of culture.

L-methionine (methyl-13C) and L-selenomethionine labeling reagents are available separately.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Line: Expi293F™
Delivery Type: Transfection
Key Functions: Protein Production
Product Line: Expi293™
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice

Contents & storage

• 3 x 0.9 mL ExpiFectamine 293 Transfection Reagent, store at 2–8°C
• 5 mL ExpiFectamine 293 Enhancer 1, store at 2–8°C
• 50 mL ExpiFectamine 293 Met (–) Enhancer 2, store at 2–8°C
• 100 mL Opti-Plex Complexation Buffer, store at 2–8°C (protect from light)
• 1 L Expi293 Met (–) Expression Medium, store at 2–8°C (protect from light)