BenchStable™ DMEM/F12

Description

Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

BenchStable DMEM/F-12 has been engineered for storage at room temperature while supporting cellular proliferation and maximum cell densities comparable to conventional DMEM when supplemented with ≥10% fetal bovine serum (FBS).

This DMEM/F-12 is modified as follows:
• Optimized for room temperature stability
• With GlutaMAX, Phenol Red
• Without HEPEPS

Using BenchStable DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. BenchStable DMEM/F-12 contains no proteins, lipids, or growth factors and so requires supplementation with ≥10% fetal bovine serum (FBS).

cGMP manufacturing and quality system
Gibco BenchStable media is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Specifications

Glutamine:	GlutaMAX™-I
Phenol Red Indicator:	Phenol Red
Form:	Liquid
HEPES Buffer:	No HEPES
Sodium Pyruvate Additive:	Sodium Pyruvate
Serum Supplementation:	Standard Serum Supplementation
Glucose:	High Glucose
Product Line:	BenchStable™
Product Size:	10 x 500 mL
Shipping Condition:	Room Temperature

Contents & storage