Interleukin 1 Beta

Catalog number:  A42507

Related applications:

Protein Assays and Analysis

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Description

Interleukin 1, beta (IL1B), a recombinant human protein, is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as immunogens, protein standards, and in other cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human IL1B (NP_000567.1) (Ala 117-Ser 269).


Activity:
1.Measured by its binding ability in a functional ELISA.
a. Immobilized recombinant human IL1B at 2 µg/ml (100µl/well) can bind recombinant human IL1R2 with a linear range of 6.25-400 ng/ml.

b. Immobilized human IL1B at 10 µg/ml (100 µl/well) can bind human IL1R1, The EC50 of human IL1R1 is 0.48 µg/ml.

2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 0.5-5 pg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Expression: E. coli
Form: Lyophilized
Gene Aliases: IL-1, IL1-BETA, IL1F2, IL1B
Gene ID: 3553
Gene Symbol: IL1B
Molecular Weight: 17 kDa
Product Size: 5 x 5 µg
Protein Family: Cytokines & Receptors
Protein Name: interleukin 1, beta
Protein Tag or Fusion: Untagged
Purity: ≥ 90% by SDS-PAGE
Research Category: Cell Cycle & Proliferation,Immunology,Oncology
Species: Human
Type: CatalogProductData
Validated Application: Assay Standard

Contents & storage

Includes 5 vials of protein in suspension buffer. Store at -20 °C.