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Invitrogen
Human PD-1 His-tag Fc Chimera Recombinant Protein
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Product Details
A42530
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
HEK293 cells
Amino acid sequence
Human PD1, amino acids Met1-Gln167 (Accession # NP_005009.2) with a C-terminal His-tagged human IgG1 Fc-tag
Tag
Fc-tag, His-tag
Molecular weight
44 kDa
Class
Recombinant
Type
Protein
Purity
>97% by SDS-PAGE
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human PD-L2 at 1 µg/mL (100 µL/well) can bind human PD1/Fc chimera with a linear range of 7.8-1000 ng/mL.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD279; CD279; PD-1; hPD1; PD-1; PD1; alternatively spliced; PDCD1; programmed cell death 1 protein; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279; systemic lupus erythematosus susceptibility 2; unnamed protein product
Gene Aliases: AIMTBS; CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; PDCD1; SLEB2
UniProt ID: (Human) Q15116
Entrez Gene ID: (Human) 5133
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