CD70 Recombinant Human Protein, hIgG1-Fc Tag

Catalog number:  A42566

Related applications:

Protein Assays and Analysis

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Description

Cluster of Differentiation 70 (CD70), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Gln 39-Pro 193) of human CD70 (NP_001243.1) fused to the Fc region of human IgG1 at the N-terminus with a polypeptide linker.

Activity: Measured by its binding ability in a functional ELISA.
1. Immobilized mouse CD27 at 2 µg/ml(100 µl/well) can bind human CD70 with a linear range of 78-1250 pg/ml.
2. Immobilized mouse CD27-Fc at 2 µg/ml(100 µl/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: CD70, CD70 / CD27L / TNFSF7, CD70 / CD27L / TNFSF7G, TNFSF7
Gene ID: 970
Gene Symbol: CD70
Molecular Weight: 45.5 kDa
Product Size: 50 µg
Protein Family: CD Proteins
Protein Form: Cell Surface Protein,Ligand,Recombinant,Truncated
Protein Name: CD70 molecule
Protein Subtype: TNF (Tumor Necrosis Factor)
Protein Tag or Fusion: IgG1 Fc Tag
Purity: ≥ 90% by SDS-PAGE
Research Category: Immunology
Species: Human
Type: CatalogProductData
Validated Application: Assay Standard

Contents & storage

Store at -20 to -70 °C