IL4 Recombinant Human Protein

Catalog number:  A42603

Related applications:

Protein Assays and Analysis

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Description

Interleukin 4 (IL-4), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the mature form of human IL4 isoform 1 (P05112-1) (His 25-Ser 153) retaining the initial Met.

Activity: Measured in a cell proliferation assay using TF1 human erythroleukemic cells (Kitamura, T. et al.,1989, J. Cell Physiol. 140:323.) . The EC50 for this effect is typically 0.1-0.3 ng/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein: interleukin 4
Expression System: E. coli
Form: Lyophilized
Protein Family: Cytokines & Receptors
Protein Form: Full Length, Recombinant
Protein Subtype: Interleukins
Research Category: Immunology, Signal Transduction
Species: Human
Validated Application: Assay Standard
Molecular Weight: 15 kDa
Purity: ≥ 90% by SDS-PAGE
Gene Alias: BCGF-1, BCGF1, BSF1, IL-4, MGC79402
Gene Symbol: IL4
Protein Tag: Untagged

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.