The Ion Torrent Oncomine BCR IGH SR Assay, DNA, is a robust, targeted next-generation sequencing (NGS) assay designed for use in hematology-oncology and minimal residual disease (MRD) monitoring research. It can also be used to investigate immunotherapy biomarkers, as well as in infectious and autoimmune disease research. The assay kit includes a single pool of multiplex PCR primers, total dNTPs, and library reagents.
The Oncomine BCR IGH SR Assay, DNA, accurately identifies and measures clonal expansion of B lymphocytes using genomic DNA from bone marrow, whole blood, peripheral blood leukocytes (PBLs), peripheral blood mononuclear cells (PBMCs), and fresh-frozen and formalin-fixed paraffin-embedded (FFPE) research samples. Using Ion AmpliSeq technology, the assay identifies unique B-cell clones through targeting of the highly diverse complementarity-determining region 3 (CDR3) of the B-cell receptor (BCR) immunoglobulin heavy (IGH) chain from a DNA template. The nucleotide sequence of the IGH CDR3 region serves as a natural barcode to enable clone identification for MRD clinical research. The measurements of B-cell clonal expansion and diversity are useful for pharmacodynamics and vaccine biomarker research. IGH CDR3-region amino acid motifs may reveal signatures of B-cell responses to defined antigens for use as future markers of autoimmune or infectious disease. For RNA samples, please see Cat. No. A45484.
Benefits of the Oncomine BCR IGH SR Assay, DNA, include: • High sensitivity and exceptionally low limit of detection for rare clone identification via dual-barcode indexing and high template capture efficiency of Ion AmpliSeq multiplex PCR • Compatibility with extracted DNA from an array of sample types, including fresh-frozen and FFPE tissue, bone marrow, whole blood, PBLs, and PBMCs • Assay primers designed to amplify all variable and joining gene alleles in gold-standard IMGT database • Generation of large libraries with minimal input requirement, facilitating study of rare clones • Detection of clones of interest at a frequency as low as 10-6 • Efficient workflow with 48-hour library-to-results time • Flexible input requirements ranging from 50 ng to 2 µg • Streamlined and user-friendly informatics solution with automated clonotyping, clonal lineage analysis, reporting of key repertoire features, and multi-sample analysis capability to generate MRD analysis for research samples • Ultra-low substitution error rate minimizing artifacts arising from sequencing errors, enabling highly accurate assessment of clonal heterogeneity in malignant B cells of interest in research samples
The Oncomine BCR IGH SR Assay, DNA, uses multiplex PCR primers to generate 90-bp amplicons that can be sequenced on all chip types supported by the Ion GeneStudio S5 sequencing systems, allowing you to pick the best multiplexing configuration for your unique sample batching needs and throughput requirements. The entire workflow from library preparation to analysis of samples can be accomplished in two days using the Ion Chef templating system and Ion GeneStudio S5 sequencing system.