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GX7 Chip and Genexus™ Coupler - FAQs

GX7 Chip and Genexus™ Coupler - FAQs

View additional product information for GX7 Chip and Genexus™ Coupler - FAQs (A46731)

15 product FAQs found

What data output sizes should I expect from the Ion Torrent GX7 Chip?

Typical sequencing output files include approximately 40 GB for uBAM files, 45 GB for BAM files, and 25 MB for VCF files. The Ion Torrent GX7 Chip generates approximately 20–25 million sequencing reads per lane for 200-base-read libraries, corresponding to approximately 80–100 million reads when all four lanes of the chip are used. Refer to the relevant Genexus Software User Guide for full details on temporary and intermediate files.

What reagents and supplies are needed with the Ion Torrent GX7 Chip and Genexus Coupler?

The Ion Torrent GX7 Chip and Genexus Coupler (Cat. No. A46731) is compatible specifically with the Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817). Refer to Ion Torrent Genexus GX7 Starter Pack-AS (Cat. No. A50222) for the full set of all core consumables required to run the Genexus Integrated Sequencer with the GX7 Chip and Genexus Coupler. However, note that quantity of Genexus consumables required depends on the assay and workflow so refer to the applicable assay or Genexus user guide for the complete materials list.

What chemistry can I use the Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) with?

The Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) can be used with Ion AmpliSeq or Ion AmpliSeq HD libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can I use the Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) with the GX5 Chip?

No, the Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) should be used with the GX7 Chip. Each kit allows for the automated template preparation and chip loading of two GX7 chips using the Genexus Integrated Sequencer.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the storage conditions for the Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817)?

The Genexus Strip 3-GX7 should be stored at 2-8 degrees C and Genexus Strip 4 should be stored at -30 to -10 degrees C.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What does the Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) consist of?

The Genexus Templating Strips 3-GX7 and 4 Kit (Cat. No. A46817) consists of 8 pairs of 3-GX7 and 4 strips, allowing for up to 2 sequencing runs per kit.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the advantages of using the GX7 Chip and Genexus Coupler (Cat. No. A46731)?

The GX7 Chip and Genexus Coupler (Cat. No. A46731) expands the capabilities of the Genexus system by generating more reads per lane than the GX5 Chip, increasing the throughput of custom Ion AmpliSeq assays and allowing the use of the Ion AmpliSeq Microbiome Health Research Assay GX.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What Genexus Software version is compatible with the GX7 Chip?

The GX7 Chip is compatible with the Genexus Software version 6.8.0 and later.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Can the Genexus Coupler be reused?

The Genexus Coupler should not be reused. The chip and coupler should be changed together.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How long can the GX7 Chip and Genexus Coupler (Cat. No. A46731) remain on the Genexus Integrated Sequencer?

The GX7 Chip and Genexus Coupler (Cat. No. A46731) can remain installed on the instrument until all lanes are used up, or within two weeks of the initial run, whichever comes first.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What are the storage conditions for the GX7 Chip and Genexus Coupler (Cat. No. A46731)?

We recommend storing GX7 Chip and Genexus Coupler (Cat. No. A46731) at room temperature.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What does the GX7 Chip and Genexus Coupler (Cat. No. A46731) consist of?

The GX7 Chip and Genexus Coupler (Cat. No. A46731) consists of two GX7 chips and two Genexus couplers, sufficient for up to 8 sequencing runs or two initializations of the Genexus Integrated Sequencer.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is the difference between GX5 and GX7 chips?

The GX7 chip provides higher throughput compared to the GX5 chip. The GX5 chip runs 12-15 million reads per lane whereas the GX7 chip runs 20-25 million reads per lane.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

How many reads can the GX7 Chip generate?

The GX7 Chip generates 20-25 million sequencing reads per lane for the 200 bp library, or 80-100 million reads per chip.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What Genexus software is needed to run GX7 chips?

You need Genexus Software v6.8 or later in order to run GX7 chips.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.