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TaqCheck™ SARS-CoV-2 Fast PCR Assay - FAQs

View additional product information for TaqCheck™ SARS-CoV-2 Fast PCR Assay - FAQs (A47693)

12 product FAQs found

Does the QuantStudio Design and Analysis Software v2.5.0 require a user license?

No, the QuantStudio Design and Analysis Software v2.5.0 is free to download using the following links: Windows OS Installer: http://downloads.thermofisher.com/Design_And_Analysis/v2.5.0/Design and Analysis Setup 2.5.0.exe Mac OS Installer: http://downloads.thermofisher.com/Design_And_Analysis/v2.5.0/Design and Analysis-2.5.0.dmg Release Notes: http://downloads.thermofisher.com/Design_And_Analysis/v2.5.0/100097775_RN_Design_And_Analysis_Software_v2.5.0.pdf

How can I obtain QuantStudio Design and Analysis Software v2.5.0 for use with the TaqCheck SARS-CoV-2 Fast PCR Assay?

Please contact our Technical Support team at techsupport@thermofisher.com to obtain QuantStudio Design and Analysis Software v2.5.0.

In the TaqCheck SARS-CoV-2 Fast PCR Assay, which reporter dyes are used and for what targets?

The FAM reporter dye is used for the RNase P target and the VIC reporter dye is used for both the SARS-CoV-2 N protein and SARS-CoV-2 S protein gene targets.

Is there a user guide associated with the TaqCheck SARS-CoV-2 Fast PCR Assay?

Yes, a Quick Reference Card is available now (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0019744_TaqCheckSARS-CoV-2_FastPCRAssay_QR.pdf). The user guide will be available by the end of March 2021 at thermofisher.com/taqcheck.

With the TaqCheck SARS-CoV-2 Fast PCR Assay, how many samples can I process in one run?

You can process 382 samples in one run, plus one positive and one negative (no-template) control per plate.

For the TaqCheck SARS-CoV-2 Fast PCR Assay, does each individual component have its own expiration date, or does the entire kit have the same expiration date?

Each individual component will have its own expiration date.

For the TaqCheck SARS-CoV-2 Fast PCR Assay, which extraction kit should I use to extract RNA from the sample?

An extraction kit is not needed. The raw saliva sample goes direct to PCR, so there is no need for a nucleic acid extraction step.

Why is the TaqCheck SARS-CoV-2 Fast PCR Assay a "Fast PCR" assay?

The TaqCheck SARS-CoV-2 Fast PCR Assay is called a "Fast PCR" assay because the PCR step utilizes modified thermal cycling conditions that take approximately 43 min - a time savings of 40% or more compared to standard PCR cycling conditions.

What comes in the TaqCheck SARS-CoV-2 Fast PCR Assay? Is the master mix included, or does it need to be purchased separately?

The TaqCheck SARS-CoV-2 Fast PCR Assay kit contains multiplexed assays that contain primer and probe sets specific to the following targets:
- SARS-CoV-2 N protein
- SARS-CoV-2 S protein
- Human RNase P RPP30

Other required components that are not included in the kit are:
- TaqCheck SARS-CoV-2 Control (Cat. No. 956127) - RNA control that contains targets specific to the SARS-CoV-2 genomic regions targeted by the assays
- TaqCheck SARS-CoV-2 Control Dilution Buffer (Cat. No. A50486) - dilution buffer for the control
- TaqPath 1-Step RT-qPCR Master Mix, CG (Cat. No. A15299 or A15300)

Which specimen types can be used with the TaqCheck SARS-CoV-2 Fast PCR Assay?

The TaqCheck SARS-CoV-2 Fast PCR Assay is currently verified for use with raw saliva.

Which instruments can be used with the TaqCheck SARS-CoV-2 Fast PCR Assay?

The instrument that is recommended for this assay is the 384-well QuantStudio 5 Real-Time PCR System. Customers who want to use other instruments will need to verify those instruments in their lab.

Which instruments, consumables, reagents, and software are required to run the TaqCheck SARS-CoV-2 Fast PCR Assay protocol? Where can I find the full list of products and protocols?

Go to thermofisher.com/taqcheck to see the workflow outlining the instruments, consumables, reagents, and software required to run the TaqCheck SARS-CoV-2 Fast PCR Assay protocol.