Applied Biosystems™

TaqCheck™ SARS-CoV-2 Fast PCR Assay

Catalog number: A47693
Applied Biosystems™

TaqCheck™ SARS-CoV-2 Fast PCR Assay

Catalog number: A47693
Catalog Number
A47693
Unit Size
1200 reactions
Price (USD)
Catalog NumberUnit SizePrice (USD)
A476931200 reactions
Product Overview
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The AppliedBiosystems TaqCheck SARS-CoV-2 Fast PCR Assay Kit is a multiplexed, highly sensitive, fast RT‐PCR assay for the qualitative detection and characterization of SARS-CoV-2 RNA (S and N genes) that compensates for emerging variants and mutations plus a human RNAse P assay to help assess sample adequacy. The SARS-CoV-2 assays target two different viral genomic regions, reducing the risk of failure to detect SARS-CoV-2. The SARS-CoV-2 N and S gene assay targets have undergone bioinformatic selection and analysis to specifically target sequences that are unique to SARS-CoV-2. The assays are designed to work with raw saliva from research samples.

The kit is used with the TaqCheck SARS-CoV-2 Control (Cat. No. 956127), TaqCheck SARS-CoV-2 Control Dilution Buffer (Cat. No. A50486), and TaqPath 1-Step RT-qPCR Master Mix, CG (Cat. No A15299 or Cat. No. A15300) (all available separately) to monitor assay-specific amplification.

Features of the TaqCheck SARS-CoV-2 Fast PCR Assay include:
Fast—helps increase speed from collection to results, enabling high-throughput testing
Cost effective—minimizes infrastructure, supplies, reagents, and personnel costs across workflow
Easy to use—simplifies workflow from raw saliva collection direct to PCR
• Multi-target design—compensates for emerging SARS-CoV-2 variants and mutations to provide confidence in results while meeting sensitivity and specificity standards

The TaqCheck SARS-CoV-2 Fast PCR Assay includes the following components:
• TaqCheck SARS-CoV-2 Fast PCR Assay—multiplexed assays that contain primer and probe sets specific to SARS-CoV-2 N gene (VIC dye), SARS-CoV-2 S gene (VIC dye), Human RNAse P RPP30 gene (FAM dye)

Required materials not supplied:
TaqCheck SARS -CoV-2 Control—RNA control that contains targets specific to the SARS-CoV-2 genomic regions targeted by the assays
TaqCheck SARS-CoV-2 Control Dilution Buffer—dilution buffer for the control
TaqPath 1 Step RT-qPCR Master Mix, CG

We have combined Thermo Fisher’s market-leading qPCR testing platform with an easy-to-use solution that brings reliability and accuracy along with key features needed for high volume SARS-CoV-2 surveillance testing. The kit can be used for medium- to large-scale testing to quickly run  96 or 384 reactions with a fast turnaround time.

The kit is validated for use with saliva directly, without any nucleic acid extraction, reducing workflow complexity and cost. The kit is a high-throughput (1,200 reactions) kit and is recommended for use with the following instruments:

• Applied Biosystems 7500 Fast Real Time PCR System, 96 well
• QuantStudio 5 Real Time PCR System, 96 well, 0.2 mL 
• QuantStudio 5 Real Time PCR System, 384 well
• QuantStudio 7 Flex Real-Time PCR System, 384 well

The assay is supported by the associated QuantStudio Design and Analysis Software 2.5.

QuantStudio Design and Analysis Software 2.5 Windows Installer (.exe)
QuantStudio Design and Analysis Software 2.5 Mac Installer (.dmg)
QuantStudio Design and Analysis Software 2.5 Release Notes (.pdf)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment)
QuantStudio™ 5 Real‐Time PCR Instrument, 384-well; 7500 Fast Real Time PCR Instrument, 96 well; QuantStudio™ 5 Real Time PCR Instrument, 96 well, 0.2-mL block; QuantStudio™ 7 Flex Real-Time PCR Instrument, 384 well
Product Line
TaqCheck™
Quantity
1200 reactions
Shipping Condition
Dry Ice
Species
Human
Target
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Validated Application
Real-Time PCR
PCR Method
1-Step RT-PCR
Reaction Speed
Fast

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