How should I reconstitute Efficient-Pro AGT Medium (Cat. No. A5322301, A5322302, A5322303, A5322304)?
Please follow the steps shown below to reconstitute Efficient-Pro AGT Medium (Cat. No. A5322301, A5322302, A5322303, A5322304) and to make Complete Medium:
- Measure 90% of the final volume of deionized or distilled water at room temperature (15-30 degrees C).
- Add Efficient-Pro AGT Medium at 27.3 grams/L to water.
- Mix for a minimum of 20 min.
- Use a calibrated vessel to dilute to final production volume with ambient deionized or distilled water.
- Mix for an additional 20 min.
- Measure the pH, then measure and record osmolality.
- Sterilize the product immediately by membrane filtration(positive pressure recommended).
Note: Once the product is filtered, use immediately or store at 2-8 degrees C for up to 6 months. Protect from light.
To make Complete Medium,
- Supplement Efficient-Pro AGT Medium with L‑glutamine or GlutaMAX Supplement at 2-8 mM final concentration prior to use (except for CHO-K1 GS cell lines).
Note: The recommended L‑glutamine and/or GlutaMAX Supplement concentration is 6 mM.
- Add 10 mL/L of HT Supplement for use in applications not requiring DHFR amplification.
- Glucose supplementation may be required for terminal batch cultures and should be determined empirically.
- Add Anti-Clumping Agent (1 mL/L) to the medium to reduce cell aggregation, if required.
Note: Consider reducing L‑glutamine concentration for fed-batch or perfusion protocols, or if the cell line in-use is sensitive to ammonia. Addition of a surfactant such as Pluronic™ F-68 is not required.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
- Measure 90% of the final volume of deionized or distilled water at room temperature (15-30 degrees C).
- Add Efficient-Pro AGT Medium at 27.3 grams/L to water.
- Mix for a minimum of 20 min.
- Use a calibrated vessel to dilute to final production volume with ambient deionized or distilled water.
- Mix for an additional 20 min.
- Measure the pH, then measure and record osmolality.
- Sterilize the product immediately by membrane filtration(positive pressure recommended).
Note: Once the product is filtered, use immediately or store at 2-8 degrees C for up to 6 months. Protect from light.
To make Complete Medium,
- Supplement Efficient-Pro AGT Medium with L‑glutamine or GlutaMAX Supplement at 2-8 mM final concentration prior to use (except for CHO-K1 GS cell lines).
Note: The recommended L‑glutamine and/or GlutaMAX Supplement concentration is 6 mM.
- Add 10 mL/L of HT Supplement for use in applications not requiring DHFR amplification.
- Glucose supplementation may be required for terminal batch cultures and should be determined empirically.
- Add Anti-Clumping Agent (1 mL/L) to the medium to reduce cell aggregation, if required.
Note: Consider reducing L‑glutamine concentration for fed-batch or perfusion protocols, or if the cell line in-use is sensitive to ammonia. Addition of a surfactant such as Pluronic™ F-68 is not required.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.