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Bethyl Laboratories
YTHDF2 Recombinant Rabbit Monoclonal Antibody (BLR297M)
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FIGURE: 1 / 4
YTHDF2 Antibody (A700-297) in WB
Detection of human YTHDF2 by western blot. Samples: Whole cell lysate (50 µg) from CCRF-CEM, MOLT-4, K-562, Jurkat, and HEL 92.1.7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-YTHDF2 recombinant monoclonal antibody [BLR297M] (A700-297 lot 1) used at 1:1,000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A700-297
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR297M
Immunogen
Recombinant antibody was purified from cell culture supernatant. Immunogen was a peptide representing a region between residue 300 and 350 of human YTH domain-containing family protein 2 using the numbering given in entry NP_057342.2 (Gene ID 51441).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
500 µg/mL
Amount
50 µg
Purification
purified
Storage buffer
PBS, pH 6.8-7.4, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation. Also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 9430020E02Rik; CLL-associated antigen KW-14; high-glucose-regulated protein 8; renal carcinoma antigen NY-REN-2; unnamed protein product; YTH domain family 2; YTH domain family, member 2; YTH N(6)-methyladenosine RNA binding protein 2; YTH N6-methyladenosine RNA binding protein 2
Gene Aliases: 9430020E02Rik; CAHL; DF2; HGRG8; NY-REN-2
UniProt ID: (Human) Q9Y5A9, (Mouse) Q91YT7
Entrez Gene ID: (Human) 51441, (Mouse) 213541
oocyte maturation
mRNA catabolic process
humoral immune response
gamete generation
spermatogonial cell division
regulation of cell adhesion
stress granule assembly
regulation of mRNA stability
negative regulation of Notch signaling pathway
positive regulation of translational initiation
embryonic morphogenesis
regulation of neurogenesis
negative regulation of type I interferon-mediated signaling pathway
mRNA destabilization
organelle assembly
hematopoietic stem cell proliferation
endothelial to hematopoietic transition
regulation of hematopoietic stem cell differentiation
regulation of meiotic cell cycle process involved in oocyte maturation
positive regulation of cap-independent translational initiation
regulation of rRNA processing
negative regulation of stem cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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