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Bethyl Laboratories

p16INK4a Recombinant Rabbit Monoclonal Antibody (BLR318M)

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p16INK4a Recombinant Rabbit Monoclonal Antibody (BLR318M)

Product Details

A700-318

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-

Flow Cytometry (Flow)

0.5 µL/1x10^6 cells
-

Immunoprecipitation (IP)

12 µL/mg
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

BLR318M

Immunogen

Recombinant antibody was purified from cell culture supernatant. Immunogen was a peptide representing a region between residue 106 and the C-terminus (residue 156) of human Cyclin-dependent kinase inhibitor 2A isoform p16INK4a using the numbering given in entry NP_000068.1 (Gene ID 1029).
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

500 µg/mL

Amount

50 µg

Purification

purified

Storage buffer

PBS, pH 6.8-7.4, with 0.1% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: a frameshift between exon 1 (0.18) and exon 2 changed the ORF of p16INK4 gene.; Alternative reading frame; ARF; CDK4 inhibitor p16 INK4; CDK4 inhibitor p16-INK4; CDK4I; CDKN2A/ARF Intron 2 lncRNA; cell cycle negative regulator beta; Cyclin dependent kinase 4 inhibitor A; Cyclin-dependent kinase 4 inhibitor A; Cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4); HGNC:1787; hypothetical 18.1 kDa protein from longest open reading frame; inhibitor of cdk4 A; inhibits CDK4; isoforms 1/2/3; Melanoma p16 inhibits CDK4; MTS-1; Multiple tumor suppressor 1; multiple tumour suppressor 1; P14 P16; p14ARF; p16; p16 gamma; p16-INK4; p16-INK4a; P19 TP16; Tumor suppressor ARF

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Gene Aliases: ARF; CAI2; CDK4I; CDKN2; CDKN2A; CMM2; INK4; INK4A; MLM; MTS-1; MTS1; P14; P14ARF; P16; P16-INK4A; P16INK4; P16INK4A; P19; P19ARF; TP16

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UniProt ID: (Human) P42771

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Entrez Gene ID: (Human) 1029

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Function(s)
p53 binding DNA binding RNA binding cyclin-dependent protein serine/threonine kinase inhibitor activity protein binding SUMO transferase activity protein kinase binding NF-kappaB binding ligase inhibitor activity ubiquitin-protein transferase inhibitor activity RNA polymerase II sequence-specific DNA binding transcription factor binding MDM2/MDM4 family protein binding disordered domain specific binding ubiquitin ligase inhibitor activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination mitophagy negative regulation of cell-matrix adhesion rRNA processing apoptotic process Ras protein signal transduction negative regulation of cell proliferation epidermis development rRNA transcription regulation of gene expression positive regulation of gene expression protein sumoylation keratinocyte differentiation negative regulation of cell growth nuclear body organization negative regulation of B cell proliferation regulation of protein stability protein destabilization negative regulation of immature T cell proliferation in thymus positive regulation of protein sumoylation mammary gland epithelial cell proliferation negative regulation of mammary gland epithelial cell proliferation positive regulation of smooth muscle cell apoptotic process protein localization to nucleus somatic stem cell population maintenance glucose homeostasis positive regulation of apoptotic process positive regulation of DNA damage response, signal transduction by p53 class mediator keratinocyte proliferation negative regulation of cyclin-dependent protein serine/threonine kinase activity negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of nucleocytoplasmic transport regulation of protein export from nucleus somatic stem cell division protein stabilization regulation of cell cycle mitochondrial depolarization apoptotic process involved in mammary gland involution positive regulation of apoptotic process involved in mammary gland involution cellular response to hydrogen peroxide protein K63-linked ubiquitination cellular senescence replicative senescence oncogene-induced cell senescence apoptotic signaling pathway positive regulation of protein localization to nucleus positive regulation of signal transduction by p53 class mediator protein localization to nucleolus negative regulation of proteolysis involved in cellular protein catabolic process regulation of protein targeting to mitochondrion negative regulation of ubiquitin protein ligase activity amyloid fibril formation regulation of G1/S transition of mitotic cell cycle negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of macrophage apoptotic process negative regulation of protein neddylation
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