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Bethyl Laboratories
IL-13 Recombinant Rabbit Monoclonal Antibody (BLR329M)
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FIGURE: 1 / 3
IL-13 Antibody (A700-329) in WB
Detection of human IL-13 by western blot. Samples: Whole cell lysate (5 µg) from 293F cells overexpressing human IL-13 treated with PNGase F deglycosylase (+) or mock treated (-). Antibody: Rabbit anti-IL-13 recombinant monoclonal antibody [BLR329M] (A700-329) used at 1:1,000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A700-329
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR329M
Immunogen
Recombinant antibody was purified from cell culture supernatant. Immunogen was a recombinant protein representing human Interleukin 13 (residues 35-146, NP_002179.2).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS, pH 6.8-7.4
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; ILN; Interleukin; Interleukin13; MGC116786; MGC116788; MGC116789; NC30; NC30 antibody; P600 antibody; T-cell activation protein P600
Gene Aliases: IL-13; P600
UniProt ID: (Human) P35225, (Mouse) P20109
Entrez Gene ID: (Human) 3596, (Mouse) 16163
microglial cell activation
positive regulation of immunoglobulin production
inflammatory response
immune response
transforming growth factor beta receptor signaling pathway
JAK-STAT cascade
response to nematode
regulation of proton transport
response to selenium ion
positive regulation of gene expression
positive regulation of B cell proliferation
positive regulation of interleukin-10 production
transforming growth factor beta1 production
positive regulation of NAD(P)H oxidase activity
response to nicotine
interleukin-13-mediated signaling pathway
macrophage activation
positive regulation of tyrosine phosphorylation of STAT protein
positive regulation of macrophage activation
positive regulation of ion transport
positive regulation of mast cell degranulation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
positive regulation of protein secretion
negative regulation of inflammatory response
positive regulation of inflammatory response
positive regulation of release of sequestered calcium ion into cytosol
cellular response to cytokine stimulus
negative regulation of transforming growth factor beta production
positive regulation of cold-induced thermogenesis
negative regulation of complement-dependent cytotoxicity
positive regulation of pancreatic stellate cell proliferation
negative regulation of endothelial cell apoptotic process
apoptotic process
response to mechanical stimulus
response to lipopolysaccharide
regulation of transforming growth factor beta1 production
response to ethanol
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
cellular response to mechanical stimulus
negative regulation of lung ciliated cell differentiation
positive regulation of lung goblet cell differentiation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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