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Bethyl Laboratories
Cytokeratin 5/6 Recombinant Rabbit Monoclonal Antibody (BLR340M)
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FIGURE: 1 / 3
Cytokeratin 5/6 Antibody (A700-340) in WB
Detection of human Cytokeratin 5/6 by western blot. Samples: Whole cell lysate (10 µg) from MCF-7, MCF-10A, RT-4, CAL-27, and T-47D cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Cytokeratin 5/6 recombinant monoclonal antibody [BLR340M] (A700-340) used at 1:1,000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A700-340
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR340M
Immunogen
Recombinant antibody was purified from cell culture supernatant. Immunogen was a peptide representing a region between residue 540 and the C-terminus (540-590) of human Cytoskeletal Keratin 5 using the numbering given in entry NP_000415.2 (Gene ID 3852).
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
40 µg/mL
Amount
4 µg
Purification
purified
Storage buffer
PBS, pH 6.8-7.4, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 58 kda cytokeratin; CK-5; CK-6C; CK-6E; CK5/6; cytokeratin 6A; cytokeratin 6B; cytokeratin 6C; cytokeratin 6D; cytokeratin-5; cytokeratin-6C; cytokeratin-6E; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K6C; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 6A, , type II; keratin 6A, type II; keratin 6B, type II; keratin 6C, type II; keratin 6E; keratin K5; keratin K6h; keratin type II; keratin, epidermal type II, K6A; keratin, epidermal, type II, K6B; keratin-like 1 (a type II keratin sequence); KRT5; type-II keratin Kb10; type-II keratin Kb12; type-II keratin Kb5; type-II keratin Kb6; unnamed protein product
Gene Aliases: CK-6B; CK-6C; CK-6E; CK5; CK6A; CK6B; CK6C; CK6D; DDD; DDD1; EBS1; EBS2; EBS2A; EBS2B; EBS2C; EBS2D; EBS2E; EBS2F; K5; K6A; K6B; K6C; K6D; K6E; KRT5A; KRT6C; KRT6D; KRT6E; KRTL1; PC2; PC3; PC4; PPKNEFD
UniProt ID: (Human) P13647, (Human) P02538, (Human) P04259, (Human) P48668
Entrez Gene ID: (Human) 3852, (Human) 3853, (Human) 3854, (Human) 286887
ectoderm development
negative regulation of cell migration
keratinization
intermediate filament organization
intermediate filament cytoskeleton organization
morphogenesis of an epithelium
positive regulation of cell proliferation
cell differentiation
killing of cells of other organism
wound healing
defense response to Gram-positive bacterium
antimicrobial humoral immune response mediated by antimicrobial peptide
negative regulation of entry of bacterium into host cell
epidermis development
response to mechanical stimulus
regulation of cell migration
regulation of protein localization
intermediate filament polymerization
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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