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Bethyl Laboratories
gamma-H2AX Recombinant Rabbit Monoclonal Antibody (BLR053F)
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FIGURE: 1 / 6
gamma-H2AX Antibody (A700-053) in ICC/IF
Detection of human gamma-H2AX by immunocytochemistry. Sample: FFPE section of human HeLa cells treated with etoposide. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody [BLR053F] (Product # A700-053) (lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A700-053
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR053F
Immunogen
Peptide surrounding phosphorylated serine 140 of human histone H2AX.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
100 µg/mL
Amount
10 µg
Purification
purified
Storage buffer
BBS, pH 7.8 to 8.6, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
H2AX is a variant of the H2A protein family and is a component of the histone octomer in nucleosomes. H2AX is phosphorylated by kinases such as ataxia telangiectasia mutated (ATM) and ATM-Rad3-related (ATR) in the PI3K pathway. Gamma-H2AX is the first step in recruiting and localizing DNA repair proteins. DSBs can be induced by mechanisms such as ionizing radiation or cytotoxic agents and subsequently, gamma-H2AX foci quickly form. These foci represent the DSBs in a 1:1 manner and can be used as a biomarker for damage. The visualization of gamma-H2AX by can allow the assessment of DNA damage, related DNA damage proteins and DNA repair. Gamma-H2AX has applications in the detection of genomic damage caused by cytotoxic chemical agents and environmental and physical damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gamma H2A X; gamma H2A.X; gamma-H2A.X; gh2ax; H2A histone family member X; H2A histone family, member X; H2a/x; H2AFX; H2AX histone; histone 5 protein 2ax; histone H2A.x; unnamed protein product
Gene Aliases: gammaH2ax; H2A.X; H2A/X; H2AFX; Hist5-2ax
UniProt ID: (Human) P16104, (Mouse) P27661
Entrez Gene ID: (Human) 3014, (Mouse) 15270
DNA damage checkpoint
double-strand break repair via homologous recombination
DNA repair
cellular response to DNA damage stimulus
spermatogenesis
heterochromatin assembly
protein K63-linked ubiquitination
cellular response to gamma radiation
positive regulation of double-strand break repair via homologous recombination
protein localization to site of double-strand break
double-strand break repair
nucleosome assembly
response to ionizing radiation
positive regulation of DNA repair
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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