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Bethyl Laboratories
ZEB1 Recombinant Rabbit Monoclonal Antibody (BLR102H)
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FIGURE: 1 / 4
ZEB1 Antibody (A700-102) in WB
Detection of mouse ZEB1 by western blot. Samples: Whole cell lysate (50 µg) from C2C12, 70Z/3, BW5147.3, CTLL-2, EL4, RenCa, NIH 3T3, TCMK-1, A20, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-ZEB1 recombinant monoclonal antibody [BLR102H] (Product # A700-102 lot 1) used at 1:1,000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody (A304-523A).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A700-102
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
BLR102H
Immunogen
Peptide representing a region between residue 1074 and the C-terminus (residue 1124) of human zinc finger E-box-binding homeobox 1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
100 µg/mL
Amount
10 µg
Purification
purified
Storage buffer
BBS, pH 7.8 to 8.6, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
ZEB1 (Zinc finger E-box-binding homeobox 1) acts as a transcriptional repressor. It inhibits interleukin-2 (IL-2) gene expression. ZEB1 enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and depending on the cell type. It represses E-cadherin promoters and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. ZEB1 also represses BCL6 transcription in the presence of the corepressor CTBP1. It postively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis. Mutations in the gene can result in corneal dystrophy, posterior polymorphous and corneal dystrophy, Fuchs endothelial 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: delta-crystallin enhancer binding factor 1; DELTA-EF1; MGC133261; negative regulator of IL2; NIL-2-A; NIL-2A; OTTHUMP00000019405; posterior polymorphous corneal dystrophy 3; RP11-472N13.4; transcription factor 8; transcription factor 8 (represses interleukin 2 expression); twirler; unnamed protein product; zinc finger homeobox protein 1a; zinc finger homeodomain enhancer-binding protein
Gene Aliases: 3110032K11Rik; [delta]EF1; AREB6; BZP; DELTAEF1; FECD6; MEB1; Nil2; NIL2A; PPCD3; TCF-8; Tcf18; TCF8; Tw; ZEB; ZFHEP; ZFHX1A; Zfx1a; Zfx1ha
UniProt ID: (Human) P37275, (Mouse) Q64318
Entrez Gene ID: (Human) 6935, (Mouse) 21417
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
E-box binding
double-stranded DNA binding
sequence-specific DNA binding
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter
central nervous system development
negative regulation of endothelial cell differentiation
positive regulation of neuron differentiation
negative regulation of transcription, DNA-templated
pattern specification process
negative regulation of cell proliferation
regulation of mesenchymal cell proliferation
regulation of transforming growth factor beta receptor signaling pathway
negative regulation of epithelial cell differentiation
regulation of T cell differentiation in thymus
positive regulation of transcription from RNA polymerase II promoter
animal organ development
embryonic camera-type eye morphogenesis
embryonic morphogenesis
embryonic skeletal system morphogenesis
semicircular canal morphogenesis
regulation of smooth muscle cell differentiation
cartilage development
cellular response to amino acid stimulus
cochlea morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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