ABsolute Blue QPCR Mix, SYBR Green, low ROX

Catalog number: AB4322B

Thermo Scientific™  Related applications: Real Time PCR (qPCR)

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Description

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Alternative Product: Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of ABsolute Blue QPCR Mix, SYBR Green, and added additional capabilities for your gene expression analysis.

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Thermo Scientific ABsolute Blue qPCR SYBR Green Mixes are optimized 2X master mixes for SYBR Green chemistry. The master mixes incorporate an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof. Absolute Blue qPCR Mixes include Thermo-Start Taq hot start enzyme for increased sensitivity.

Highlights

High performance—ABsolute sensitivity, consistency & convenience
Enhanced contrast and reduced errors—easily identify correctly aliquotted reaction mix in all plates and tubes
Universal—compatible with all major qPCR platforms
Adaptable—available with ROX or Fluorescein passive reference dyes

Applications

• Gene expression analysis
• siRNA validation
• Genotyping

Includes
• ABsolute Blue qPCR SYBR Green low ROX Mix contains 2X master mix supplemented with low ROX reference dye concentration

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: SYBR
For Use With (Equipment): 7500 System, QuantStudio™, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, ViiA™ 7 System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA, dsDNA
Number of Reactions: 1,600 Reactions
Polymerase: Taq DNA Polymerase
Product Line: ABsolute Blue
Product Size: 1,600 reactions

Contents & storage

Contains:
• 16 x 1.25 mL ABsolute Blue QPCR SYBR Green Low ROX Mix (2X)
• 100 µL MgCl2 (1M)
Sufficient for 1600 x 25 µL reactions.

Store at -20°C

Documents

Manuals & protocols

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