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c-Myc Monoclonal Antibody (9E10.3)
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FIGURE: 1 / 6
c-Myc Antibody (AHO0062) in IHC (P)
Immunohistochemical analysis of c-Myc in formalin-fixed, paraffin-embedded human breast carcinoma using a c-Myc monoclonal antibody (Product # AHO0062). Detection was performed with a peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
AHO0062
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse,
Plant,
Tag,
Yeast
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
9E10.3
Immunogen
A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc, coupled to KLH.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects an epitope at aa 410-419.
Staining of formalin-fixed paraffin embedded tissue sections requires boiling the sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes. Recommended positive controls include Raji cells and human breast carcinoma.
Target Information
The c-Myc protein is a 62 kDa transcription factor that is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies have shown that c-Myc is essential for vasculogenesis and angiogenesis in tumor development, which distributes blood throughout the cells. The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation, and the deregulated expression of c-Myc induces apoptosis in different cell types. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins. c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. Translocation of the c-myc locus on chromosome 8 to the immunoglobulin loci on chromosome 14 (heavy chain); 2 (delta light chain); or 22 (light chain) is described in Burkitts lymphoma and other B-cell lymphoproliferative conditions. An aberrant expression of the c-myc gene occurs in tumors of different origins such as colorectal, gastric, gallbladder, hepatic, mammary, ovarian, endometrial, head and neck, pulmonary, prostatic, thyroidal, oral, ocular, nasopharyngeal, endocrine, as well as hematopoietic neoplasms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: avian myelocytomatosis viral oncogene homolog; c Myc; c-myc; c-myc epitope tag; c-myc protein, first expressed; class E basic helix-loop-helix protein 39; cmyc; Myc protein; myc-related translation/localization regulatory factor; ORF 114; ORF 114; putative; ORF 118; proto-oncogene c-Myc; transcription factor p64; unnamed protein product; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog
Gene Aliases: bHLHe39; c-Myc; MRTL; MYCC
UniProt ID: (Human) P01106
Entrez Gene ID: (Human) 4609
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter sequence-specific DNA binding
transcription cofactor binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
macromolecular complex binding
protein dimerization activity
E-box binding
DNA-binding transcription factor binding
SCF ubiquitin ligase complex binding
G1/S transition of mitotic cell cycle
negative regulation of transcription from RNA polymerase II promoter
MAPK cascade
branching involved in ureteric bud morphogenesis
positive regulation of mesenchymal cell proliferation
chromatin remodeling
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular iron ion homeostasis
cellular response to DNA damage stimulus
positive regulation of cell proliferation
response to xenobiotic stimulus
regulation of gene expression
regulation of cell cycle process
positive regulation of gene expression
rRNA metabolic process
regulation of telomere maintenance
positive regulation of telomere maintenance
negative regulation of stress-activated MAPK cascade
protein-DNA complex disassembly
cellular response to UV
negative regulation of apoptotic process
hypermethylation of CpG island
fibroblast apoptotic process
negative regulation of monocyte differentiation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
negative regulation of fibroblast proliferation
positive regulation of epithelial cell proliferation
chromosome organization
negative regulation of cell division
negative regulation of transcription initiation from RNA polymerase II promoter
ERK1 and ERK2 cascade
response to growth factor
cellular response to hypoxia
cellular response to xenobiotic stimulus
positive regulation of metanephric cap mesenchymal cell proliferation
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
regulation of somatic stem cell population maintenance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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