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Invitrogen
INSR beta Monoclonal Antibody (CT-3)
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FIGURE: 1 / 14
INSR beta Antibody (AHR0271) in ICC/IF
Immunofluorescent analysis of Insulin Receptor beta (green) showing staining in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor beta monoclonal antibody (Product #... View More
Product Details
AHR0271
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
CT-3
Immunogen
Recombinant fragment including the C-terminal 100 amino acid residues of human insulin receptor.
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Staining of formalin-fixed paraffin embedded tissue sections requires boiling the sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Target Information
Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD220; CD220 beta; CD221; IGF-I receptor; Insulin receptor; insulin receptor precursor; IR; IR beta; unnamed protein product
Gene Aliases: 4932439J01Rik; CD220; D630014A15Rik; HHF5; INSR; IR; IR-A; IR-B
UniProt ID: (Human) P06213, (Mouse) P15208
Entrez Gene ID: (Human) 3643, (Mouse) 16337
nucleotide binding
beta-amyloid binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
insulin-activated receptor activity
insulin-like growth factor receptor binding
structural molecule activity
protein binding
ATP binding
GTP binding
kinase activity
transferase activity
protein domain specific binding
protein kinase activator activity
insulin-like growth factor I binding
insulin-like growth factor II binding
cargo receptor activity
identical protein binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
macromolecular complex binding
PTB domain binding
receptor signaling protein tyrosine kinase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
protein phosphatase binding
lipoic acid binding
protein complex binding
3-phosphoinositide-dependent protein kinase binding
transmembrane signal receptor
positive regulation of receptor internalization
heart morphogenesis
regulation of glycogen biosynthetic process
regulation of transcription, DNA-templated
receptor-mediated endocytosis
transmembrane receptor protein tyrosine kinase signaling pathway
G-protein coupled receptor signaling pathway
learning
memory
positive regulation of cell proliferation
insulin receptor signaling pathway
epidermis development
male gonad development
organ morphogenesis
regulation of gene expression
positive regulation of macromolecule biosynthetic process
regulation of glucose metabolic process
male sex determination
adrenal gland development
positive regulation of cell migration
exocrine pancreas development
receptor internalization
cellular response to insulin stimulus
glucose homeostasis
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of protein complex disassembly
positive regulation of MAPK cascade
positive regulation of nitric oxide biosynthetic process
positive regulation of glycogen biosynthetic process
positive regulation of glycolytic process
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
regulation of embryonic development
positive regulation of glucose import
viral entry into host cell
protein autophosphorylation
animal organ development
positive regulation of developmental growth
system development
positive regulation of cellular component organization
regulation of multicellular organismal process
positive regulation of meiotic cell cycle
positive regulation of protein kinase B signaling
positive regulation of respiratory burst
cellular response to growth factor stimulus
dendritic spine maintenance
beta-amyloid clearance
transport across blood-brain barrier
neuron projection maintenance
regulation of female gonad development
activation of MAPK activity
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
protein phosphorylation
regulation of hydrogen peroxide metabolic process
positive regulation of glycoprotein biosynthetic process
negative regulation of gene expression
phosphorylation
peptidyl-tyrosine phosphorylation
transformation of host cell by virus
signal transduction by protein phosphorylation
activation of protein kinase activity
activation of protein kinase B activity
negative regulation of transporter activity
response to tumor necrosis factor
intracellular signal transduction
peptidyl-tyrosine autophosphorylation
positive regulation of phosphorylation
positive regulation of DNA replication
protein heterotetramerization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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