Applied Biosystems™

MagMAX™ Total Nucleic Acid Isolation Kit

Catalog number:  AM1840

Related applications:

Animal Health

| DNA Extraction

| RNA Extraction

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The MagMAX™ Total Nucleic Acid Isolation Kit is designed for the rapid, high-throuphput purification of RNA and genomic DNA from a variety of samples including viral, blood, and bacterial samples. It includes zirconia beads for the mechanical disruption of difficult pathogens. RNA purified using the kit is ideal for PCR and RT-PCR applications. The kit uses magnetic bead–based purification and includes sufficient reagents for 100 reactions.

• Complete, ready-to-use reagents for RNA and DNA purification from tough-to-lyse bacteria
• Highly effective sample homogenization using bead beating and chemical lysis
• Magnetic bead–based purification technology allows effective removal of PCR inhibitors
• Nucleic acid purification is easily scalable and suitable for automation

Proven with a Wide Range of Samples:
• Biological Samples
• Feces and whole blood
• Biofluids such as milk, urine, nasal fluids, swab samples, semen, sputum, and spent culture medium
• Bacterial cultures and mammalian cell cultures
• Environmental Samples
• River and pond water
• Agricultural samples such as manure pits and cow alleyways

Benefits of the MagMAX™ Total Nucleic Acid Isolation Kit:
The MagMAX™ Total Nucleic Acid Isolation Kit procedure combines an effective method sample disruption with magnetic bead–based nucleic acid isolation and purification technology. The MagMAX™ microspherical paramagnetic beads have a large available binding surface and can be fully dispersed in solution, allowing thorough nucleic acid binding, washing, and elution. This results in consistent yields of high-quality nucleic acid with >50% recovery and little sample-to-sample variation (recovery may vary depending on sample type). The entire procedure can be completed in approximately 1 hour.

Overview of the Procedure:
Samples are disrupted using zirconia beads in a guanidinium thiocyanate–based solution; this rapidly releases nucleic acid while simultaneously inactivating nucleases. Samples are then diluted with isopropanol, and paramagnetic beads are added to the sample. The beads bind nucleic acids and are then immobilized on magnets and washed to remove proteins and other contaminants. A second wash solution is used to remove residual binding solution. Finally, nucleic acid is eluted in a small volume of low-salt buffer. The MagMAX™ Total Nucleic Acid Isolation Kit procedure is designed for processing samples in 96-well plates, thus 96 samples can be processed at once; however, the kit can also be used with fewer than 96 samples.

Accessory Products:
Lysis/Binding Solution Concentrate (SKU#AM8500), Wash Solution 1 Concentrate (SKU#AM8504), and Wash Solution 2 Concentrate (SKU#AM8640) are components of the MagMAX™ Total Nucleic Acid Isolation Kit that may also be purchased separately.
For Laboratory Use Including Veterinary and Environmental Uses


Final Product Type: RNA, DNA
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Southern Blotting, PCR, Northern Blotting
Isolation Technology: Magnetic Bead
No. of Reactions: 100 reactions
Purification Target: Total RNA, Total Nucleic Acids, Genomic DNA
Purification Time: 1 hrs
Sample Type: Liquid Samples (e.g. Serum), Blood, Cells, Bacteria
Format: 96-well plate
Yield: 420 ng
High-throughput Compatibility: High-throughput Compatible
Starting Material Amount: Up to 0.3 g solid samples or 175 µl liquid samples
Product Line: MagMAX™

Contents & storage

Carrier NA and Lysis⁄Binding Enhancer are stored at –20°C and RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, Processing Plates and Lids, and PBS Buffer are stored at room temperature.