MEGAclear™ Transcription Clean-Up Kit - FAQs

View additional product information for MEGAclear™ Transcription Clean-Up Kit - FAQs (AM1908)

5 product FAQs found

How do I remove DNase from an RNA sample?

DNase can be removed by the following methods: acid phenol:choloform extraction, lithium chloride precipitation, EDTA, and heat inactivation, or using our MegaClear Transcription Clean-Up Kit (Cat. No. AM1908).

Alternatively, our Invitrogen TURBO DNA-free Kit (Cat. No. AM1907) is supplied with a DNase inactivation reagent that can be used to remove the DNase, as well as divalent cations such as magnesium and calcium, which can catalyze RNA degradation when RNA is heated with the sample.

What is the binding capacity of the MEGAclear kit filter column?

The binding capacity of the 10051G filter columns is 500µg of RNA

Can the Filter Cartridges within the MEGAclear Transcription Clean-Up Kit (Cat. No. AM1908) be ordered separately?

Yes, we do offer the Spin Cartridges (Cat. No. AM10051G) as a stand-alone product, and they would be compatible with the MEGAclear Transcription Clean-Up Kit (Cat. No. AM1908).

Can the collection tubes within the MEGAclear Transcription Clean-Up Kit (Cat. No. AM1908) be ordered separately?

Yes, the Elution Tubes (2.0 mL) (Cat. No. AM12480) are the same that are used in the MEGAclear Transcription Clean-Up Kit (Cat. No. AM1908).

What is the composition of the Elution Solution provided in the MEGAclear Transcription Clean-Up Kit (Cat. No. AM1908)?

It is 0.1 mM EDTA (pH 8.0) in nuclease-free water.