Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures.
AuthorsSieracki ME, Cucci TL, Nicinski J,
JournalAppl Environ Microbiol
PubMed ID10347021
'The respiratory activity of marine bacteria is an important indication of the ecological functioning of these organisms in marine ecosystems. The redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) is reduced intracellularly in respiring cells to an insoluble, fluorescent precipitate. This product is detectable and quantifiable by flow cytometry in individual cells. ... More
Comparison of the antimicrobial effects of chlorine, silver ion, and tobramycin on biofilm.
AuthorsKim J, Pitts B, Stewart PS, Camper A, Yoon J,
JournalAntimicrob Agents Chemother
PubMed ID18195062
'The systematic understanding of how various antimicrobial agents are involved in controlling biofilms is essential in order to establish an effective strategy for biofilm control, since many antimicrobial agents are effective against planktonic cells but are ineffective when they are used against the same bacteria growing in a biofilm state. ... More
Characterization of osmotically induced filaments of Salmonella enterica.
AuthorsPratt ZL, Chen B, Czuprynski CJ, Wong AC, Kaspar CW,
JournalAppl Environ Microbiol
PubMed ID22798362
'Salmonella enterica forms aseptate filaments with multiple nucleoids when cultured in hyperosmotic conditions. These osmotic-induced filaments are viable and form single colonies on agar plates even though they contain multiple genomes and have the potential to divide into multiple daughter cells. Introducing filaments that are formed during osmotic stress into ... More
Inactivation of Escherichia coli by nanoparticulate zerovalent iron and ferrous ion.
AuthorsKim JY, Park HJ, Lee C, Nelson KL, Sedlak DL, Yoon J,
JournalAppl Environ Microbiol
PubMed ID20870787
'The mechanism of Escherichia coli inactivation by nanoparticulate zerovalent iron (nZVI) and Fe(II) was investigated using reactive oxygen species (ROS) quenchers and probes, an oxidative stress assay, and microscopic observations. Disruption of cell membrane integrity and respiratory activity was observed under deaerated conditions [more disruption by nZVI than Fe(II)], and ... More
How the insect immune system interacts with an obligate symbiotic bacterium.
AuthorsDouglas AE, Bouvaine S, Russell RR,
JournalProc Biol Sci
PubMed ID20719775
The animal immune system provides defence against microbial infection, and the evolution of certain animal-microbial symbioses is predicted to involve adaptive changes in the host immune system to accommodate the microbial partner. For example, the reduced humoral immune system in the pea aphid Acyrthosiphon pisum, including an apparently non-functional immune ... More
The cell membrane as a major site of damage during aerosolization of Escherichia coli.
This study aimed to provide data on the survival and site of damage of Escherichia coli cells following aerosolization using two different techniques, nebulization and flow focusing. Four metabolic stains were assessed for their ability to detect respiratory activities and membrane homeostasis in aerosolized E. coli cells. The degree of ... More