'OX40 is a member of the tumor necrosis factor (TNF) receptor superfamily and known to be an important costimulatory molecule expressed on activated T cells. To investigate the role of costimulation of OX40 in human immunodeficiency virus type 1 (HIV-1) infection by its natural ligand, gp34, the OX40-transfected ACH-2 cell ... More
Cytoskeletal changes regulated by the PAK4 serine/threonine kinase are mediated by LIM kinase 1 and cofilin.
Authors Dan C; Kelly A; Bernard O; Minden A;
JournalJ Biol Chem
PubMed ID11413130
'PAK4 is the most recently identified member of the PAK family of serine/threonine kinases. PAK4 differs from other members of the PAK family in sequence and in many of its functions. Previously, we have shown that an important function of this kinase is to mediate the induction of filopodia in ... More
The enzymological basis for resistance of herpesvirus DNA polymerase mutants to acyclovir: relationship to the structure of alpha-like DNA polymerases.
'Acyclovir (ACV), like many antiviral drugs, is a nucleoside analog. In vitro, ACV triphosphate inhibits herpesvirus DNA polymerase by means of binding, incorporation into primer/template, and dead-end complex formation in the presence of the next deoxynucleoside triphosphate. However, it is not known whether this mechanism operates in vivo. To address ... More
Expression and characterization of functionally active recombinant perforin produced in insect cells.
AuthorsLiu CC, Persechini PM, Young JD
JournalJ Immunol
PubMed ID8617952
'A key cytolytic mediator used by killer lymphocytes, perforin (also known as pore-forming protein or cytolysin), has been shown to be capable of undergoing polymerization to form pores in cell membranes and cause osmotic lysis of target cells. Although perforin has been purified from killer lymphocytes and the coding gene ... More
NS5A, a nonstructural protein of hepatitis C virus, binds growth factor receptor-bound protein 2 adaptor protein in a Src homology 3 domain/ligand-dependent manner and perturbs mitogenic signaling.
AuthorsTan SL, Nakao H, He Y, Vijaysri S, Neddermann P, Jacobs BL, Mayer BJ, Katze MG
JournalProc Natl Acad Sci U S A
PubMed ID10318918
'Although hepatitis C virus (HCV) infection is an emerging global epidemic causing severe liver disorders, the molecular mechanisms of HCV pathogenesis remain elusive. The NS5A nonstructural protein of HCV contains several proline-rich sequences consistent with Src homology (SH) 3-binding sites found in cellular signaling molecules. Here, we demonstrate that NS5A ... More
Identification of phosphorylated residues that affect the activity of the mitotic kinase Aurora-A.
Authors Littlepage Laurie E; Wu Hua; Andresson Thorkell; Deanehan Julia K; Amundadottir Laufey T; Ruderman Joan V;
JournalProc Natl Acad Sci U S A
PubMed ID12422018
'The activity of the kinase Aurora-A (Aur-A) peaks during mitosis and depends on phosphorylation by one or more unknown kinases. Mitotic phosphorylation sites were mapped by mass spec sequencing of recombinant Aur-A protein that had been activated by incubation in extracts of metaphase-arrested Xenopus eggs. Three sites were identified: serine ... More
Identification of the major synaptojanin-binding proteins in brain.
Authorsde Heuvel E, Bell AW, Ramjaun AR, Wong K, Sossin WS, McPherson PS
JournalJ Biol Chem
PubMed ID9079704
'Synaptojanin is a nerve-terminal enriched inositol 5-phosphatase thought to function in synaptic vesicle endocytosis, in part through interactions with the Src homology 3 domain of amphiphysin. We have used synaptojanin purified from Sf9 cells after baculovirus mediated expression in overlay assays to identify two major synaptojanin-binding proteins in rat brain. ... More
Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer.
'Prolyl 4-hydroxylase (proline hydroxylase, EC 1.14.11.2) catalyzes the formation of 4-hydroxyproline in collagens. The vertebrate enzyme is an alpha2beta2 tetramer, the beta subunit of which is identical to protein disulfide-isomerase (PDI, EC 5.3.4.1). We report here on cloning of the recently discovered alpha(II) subunit from human sources. The mRNA for ... More
Molecular cloning and characterization of Porphyromonas gingivalis lysine-specific gingipain. A new member of an emerging family of pathogenic bacterial cysteine proteinases.
'The proteinases of Porphyromonas gingivalis are key virulence factors in the etiology and progression of periodontal disease. Previous work in our laboratories resulted in the purification of arginine- and lysine-specific cysteine proteinases, designated gingipains, that consist of several tightly associated protein subunits. Recent characterization of arginine-specific gingipain-1 (gingipain R1; RGP-1) ... More
15-Lipoxygenase metabolism of 2-arachidonylglycerol. Generation of a peroxisome proliferator-activated receptor alpha agonist.
Authors Kozak Kevin R; Gupta Rajnish A; Moody John S; Ji Chuan; Boeglin William E; DuBois Raymond N; Brash Alan R; Marnett Lawrence J;
JournalJ Biol Chem
PubMed ID11956198
'The recent demonstrations that cyclooxygenase-2 and leukocyte-type 12-lipoxygenase (LOX) efficiently oxygenate 2-arachidonylglycerol (2-AG) prompted an investigation into related oxygenases capable of metabolizing this endogenous cannabinoid receptor ligand. We evaluated the ability of six LOXs to catalyze the hydroperoxidation of 2-AG. Soybean 15-LOX, rabbit reticulocyte 15-LOX, human 15-LOX-1, and human 15-LOX-2 ... More
Role of phosphorylation sites and the C2 domain in regulation of cytosolic phospholipase A2.
AuthorsGijon MA, Spencer DM, Kaiser AL, Leslie CC
JournalJ Cell Biol
PubMed ID10366595
'Cytosolic phospholipase A2 (cPLA2) mediates agonist-induced arachidonic acid release, the first step in eicosanoid production. cPLA2 is regulated by phosphorylation and by calcium, which binds to a C2 domain and induces its translocation to membrane. The functional roles of phosphorylation sites and the C2 domain of cPLA2 were investigated. In ... More
Accurate processing and secretion in the baculovirus expression system of an erythroid-cell-stimulating factor consisting of a chimaera of insulin-like growth factor II and an insect insulin-like peptide.
AuthorsCongote LF, Li Q
JournalBiochem J
PubMed ID8166627
'A synthetic gene encoding the signal peptide and the N-terminal sequence of bombyxin, an insect insulin-like peptide, and the 58 amino acids of the C-terminal sequence of human insulin-like growth factor II (IGF II) has been expressed using the baculovirus system. This synthetic chimaera was obtained by amplification of four ... More
Baculovirus expression and purification of rat 10- formyltetrahydrofolate dehydrogenase.
AuthorsKrupenko SA, Horstman DA, Wagner C, Cook RJ
JournalProtein Expr Purif
PubMed ID8527931
'The liver cytosolic enzyme, 10-formyltetrahydrofolate dehydrogenase (10- FTHFDH) (EC 1.5.1.6) catalyzes two reactions: the NADP(+)-dependent oxidation of 10-formyltetrahydrofolate to tetrahydrofolate and CO2 and the NADP(+)-independent hydrolysis of 10-formyltetrahydrofolate to tetrahydrofolate and formate. It exists as a tetramer of 99-kDa subunits in rat liver. We expressed rat liver cDNA encoding 10-FTHFDH ... More
Interaction of nucleotides with membrane-associated cystic fibrosis transmembrane conductance regulator.
AuthorsTravis SM, Carson MR, Ries DR, Welsh MJ
JournalJ Biol Chem
PubMed ID7687995
'Cystic fibrosis transmembrane conductance regulator (CFTR) is a Cl- channel that is regulated by cytosolic nucleotides and by cAMP- dependent phosphorylation. In excised membrane patches, CFTR Cl- channel activity requires hydrolyzable nucleotides and Mg2+, and is inhibited by ADP. We examined the interactions between CFTR and nucleotides using 8-azidoadenosine 5''-triphosphate ... More
Endotoxin-mediated endothelial cell injury and activation: role of soluble CD14.
AuthorsArditi M, Zhou J, Dorio R, Rong GW, Goyert SM, Kim KS
JournalInfect Immun
PubMed ID7687581
'Vascular endothelial cell (EC) injury by lipopolysaccharides (LPS) plays a major role in the pathogenesis of gram-negative bacterial sepsis and endotoxic shock. The studies described here were performed to define further the molecular mechanisms involved in the EC responses to LPS. We showed that serum was required for LPS-mediated cytotoxicity ... More
Purification, reconstitution, and steady-state kinetics of the trans-membrane 17 beta-hydroxysteroid dehydrogenase 2.
Authors Lu Ming-Liang; Huang Yi-Wei; Lin Sheng-Xiang;
JournalJ Biol Chem
PubMed ID11940569
'Human membrane 17 beta-hydroxysteroid dehydrogenase 2 is an enzyme essential in the conversion of the highly active 17beta-hydroxysteroids into their inactive keto forms in a variety of tissues. 17 beta-hydroxysteroid dehydrogenase 2 with 6 consecutive histidines at its N terminus was expressed in Sf9 insect cells. This recombinant protein retained ... More
Production of a recombinant imported fire ant venom allergen, Sol i 2, in native and immunoreactive form.
AuthorsSchmidt M, McConnell TJ, Hoffman DR
JournalJ Allergy Clin Immunol
PubMed ID8765821
'BACKGROUND: The complementary DNA encoding for the important imported fire ant venom allergen, Sol i 2, has previously been cloned. The binding of human IgE antibodies to Sol i 2 has been demonstrated to be conformation-dependent. METHODS: A couple cDNA clone encoding the Sol i 2 protein sequence and its ... More
Substitutions of glutamate 781 in the Na,K-ATPase alpha subunit demonstrate reduced cation selectivity and an increased affinity for ATP.
AuthorsKoster JC, Blanco G, Mills PB, Mercer RW
JournalJ Biol Chem
PubMed ID8576200
'The intramembrane Glu781 residue of the Na,K-ATPase alpha subunit has been postulated to have a role in the binding and/or occlusion of cations. To ascertain the role of Glu781, the residue was substituted with an aspartate, alanine, or lysine residue and the mutant Na,K- ATPases were coexpressed with the native ... More
The exoskeleton collagens in Caenorhabditis elegans are modified by prolyl 4-hydroxylases with unique combinations of subunits.
'The collagen prolyl 4-hydroxylases (P4Hs, EC ) play a critical role in the synthesis of the extracellular matrix. The enzymes characterized from vertebrates and Drosophila are alpha(2)beta(2) tetramers, in which protein disulfide isomerase (PDI) serves as the beta subunit. Two conserved alpha subunit isoforms, PHY-1 and PHY-2, have been identified ... More
Segregated coupling of phospholipases A2, cyclooxygenases, and terminal prostanoid synthases in different phases of prostanoid biosynthesis in rat peritoneal macrophages.
AuthorsNaraba H, Murakami M, Matsumoto H, Shimbara S, Ueno A, Kudo I, Oh-ishi S
JournalJ Immunol
PubMed ID9510202
'We examined herein the functional linkage of enzymes regulating the initial, intermediate, and terminal steps of PG biosynthesis to provide PGs in rat peritoneal macrophages stimulated with LPS and/or A23187. Quiescent cells stimulated with A23187 produced thromboxane B2 (TXB2) in marked preference to PGE2 within 30 to 60 min (constitutive ... More
Estimation of baculovirus titer by beta-galactosidase activity assay of virus preparations.
AuthorsYahata T, Andriole S, Isselbacher KJ, Shioda T
JournalBiotechniques
PubMed ID10948416
There is no abstract available. The title describes the paper.
Plants are attacked by a broad array of herbivores and pathogens. In response, plants deploy an arsenal of defensive traits. In Brassicaceae, the glucosinolate-myrosinase complex is a sophisticated two-component system to ward off opponents. However, this so-called ... More
Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes.
AuthorsCrawford F, Kozono H, White J, Marrack P, Kappler J
JournalImmunity
PubMed ID9655481
Multimeric soluble MHC class II molecules stably occupied with covalently attached peptides bind with appropriate specificity to T cell hybridomas and T cells from T cell receptor transgenic mice. There is a direct correlation between soluble T cell receptor affinity for monomeric MHC/peptide and level of binding of multimeric MHC/peptide ... More
The ubiquitous chromatin protein DEK alters the structure of DNA by introducing positive supercoils.
We have investigated the molecular mechanism by which the proto-oncogene protein DEK, an abundant chromatin-associated protein, changes the topology of DNA in chromatin in vitro. Band-shift assays and electron microscopy revealed that DEK induces both intra- and intermolecular interactions between DNA molecules. Binding of the DEK protein introduces constrained positive ... More
Involvement of a bifunctional fatty-acyl desaturase in the biosynthesis of the silkmoth, Bombyx mori, sex pheromone.
The straight-chain C(10) to C(18) unsaturated aliphatic compounds containing an oxygenated functional group (aldehyde, alcohol, or acetate ester) derived from saturated C(16) or C(18) fatty acids are a major class of sex pheromone components produced by female moths. In the biosynthesis of these pheromone components, various combinations of limited chain-shortening ... More
Regulation of 4E-BP1 phosphorylation: a novel two-step mechanism [In Process Citation]
AuthorsGingras AC, Gygi SP, Raught B, Polakiewicz RD, Abraham RT, Hoekstra MF, Aebersold R, Sonenberg N
JournalGenes Dev
PubMed ID10364159
The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). Binding of the ... More
Expression of rat liver phenylalanine hydroxylase in insect cells and site-directed mutagenesis of putative non-heme iron-binding sites.
AuthorsGibbs BS, Wojchowski D, Benkovic SJ
JournalJ Biol Chem
PubMed ID8385134
Rat liver phenylalanine hydroxylase was expressed in both Escherichia coli and the Spodoptera frugiperda insect cell line, Sf9. Recombinant enzyme from E. coli was inactive and contained less than 0.1 iron atom/subunit. In contrast, recombinant enzyme expressed in Sf9 cells using a baculovirus vector was active and identical in several ... More
Association of IRS-1 with the insulin receptor and the phosphatidylinositol 3'-kinase. Formation of binary and ternary signaling complexes in intact cells.
AuthorsBacker JM, Myers MG Jr, Sun XJ, Chin DJ, Shoelson SE, Miralpeix M, White MF
JournalJ Biol Chem
PubMed ID8385139
Insulin stimulates the formation of binary and ternary signaling complexes between the phosphatidylinositol (PtdIns) 3'-kinase, IRS-1, and the insulin receptor in vivo. Binary complex formation between IRS- 1 and the PtdIns 3'-kinase occurs in intact cells and requires the tyrosyl phosphorylation IRS-1, as mutant insulin receptors which weakly phosphorylate IRS-1 ... More
Use of the baculovirus system to assemble polyomavirus capsid-like particles with different polyomavirus structural proteins: analysis of the recombinant assembled capsid-like particles.
AuthorsAn K, Gillock ET, Sweat JA, Reeves WM, Consigli RA
JournalJ Gen Virol
PubMed ID10211971
The genes encoding the structural proteins (VP1, VP2 and VP3) of murine polyomavirus were cloned into the p2Bac dual multiple cloning site vector, individually or jointly, and the corresponding proteins were expressed in Spodoptera frugiperda (Sf9) insect cells by cotransfecting Sf9 cells with the constructed vector and the linear DNA ... More
Characterization of nuclear pore protein p62 produced using baculovirus.
AuthorsBailer SM, Berlin WK, Starr CM, Hanover JA
JournalProtein Expr Purif
PubMed ID8527942
Nuclear pore glycoproteins are essential components of the nuclear import apparatus in eukaryotes. In vertebrates, the most abundant of these glycoproteins is a molecule called p62. Like other O-linked N- acetylglucosamine glycoproteins, p62 is normally modified in the cytoplasm and cannot be overexpressed and conveniently collected in a secreted form. ... More
Identification of the ligand-binding regions in the macrophage colony- stimulating factor receptor extracellular domain.
AuthorsWang ZE, Myles GM, Brandt CS, Lioubin MN, Rohrschneider L
JournalMol Cell Biol
PubMed ID8355686
The c-fms gene encodes the receptor for the macrophage colony- stimulating factor (M-CSF), and its extracellular domain consists of five immunoglobulin-like subdomains. To identify which of the five immunoglobulin-like regions are involved in ligand binding, we polymerase chain reaction-cloned five segments of the extracellular domain of the murine c-fms gene, ... More
Dissection of CR1, factor H, membrane cofactor protein, and factor B binding and functional sites in the third complement component.
Previous studies have suggested that the residues 727-768 of human (Hu) C3 contain the binding sites for CR1, factor H, and factor B. Here, we have (1) characterized further some of the C3 structural requirements for its binding to CR1, H, and B, (2) investigated the functions associated with these ... More
Expression of bovine vitamin K-dependent carboxylase activity in baculovirus-infected insect cells.
AuthorsRoth DA, Rehemtulla A, Kaufman RJ, Walsh CT, Furie B, Furie BC
JournalProc Natl Acad Sci U S A
PubMed ID8378308
A vitamin K-dependent carboxylase has recently been purified from bovine liver microsomes and candidate cDNA clones have been isolated. Definitive identification of the carboxylase remains circumstantial since expression of candidate carboxylase cDNAs in mammalian cells is confounded by the presence of endogenous carboxylase activity. To overcome this problem, a recombinant ... More
Enzymatic properties of human Na,K-ATPase alpha1beta3 isozyme.
AuthorsYu C, Xie Z, Askari A, Modyanov NN
JournalArch Biochem Biophys
PubMed ID9281321
Recent results of a wide-scale human cDNA sequencing project have identified a cDNA which encodes a hitherto unknown human protein sequence exhibiting structural similarities with beta-subunits of the Na,K- and H,K-ATPase family and with the amphibian Na,KATPase beta3-subunit, in particular. In this study the ability of the putative human beta3-subunit ... More
Polyomavirus major capsid protein VP1 is capable of packaging cellular DNA when expressed in the baculovirus system
AuthorsGillock ET, Rottinghaus S, Chang D, Cai X, Smiley SA, An K, Consigli RA
JournalJ Virol
PubMed ID9060642
Using the p2Bac dual multiple cloning site transfer vector, the polyomavirus major capsid protein gene VP1 was cloned for expression in the baculovirus-insect cell expression system. The 5-day-infected cellular lysate from this recombinant preparation was purified by cesium chloride density gradient centrifugation. Capsid-like particles were observed in the resulting preparation. ... More
Heparin promotes proteolytic inactivation by thrombin of a reactive site mutant (L444R) of recombinant heparin cofactor II.
AuthorsCiaccia AV, Willemze AJ, Church FC
JournalJ Biol Chem
PubMed ID8995378
A heparin cofactor II (HCII) mutant with an Arg substituted for Leu444 at the P1 position (L444R-rHCII) was previously found to have altered proteinase specificity (Derechin, V. M., Blinder, M. A., and Tollefsen, D. M. (1990) J. Biol. Chem. 265, 5623-5628). The present study characterizes the effect of glycosaminoglycans on ... More
The Bmx tyrosine kinase induces activation of the Stat signaling pathway, which is specifically inhibited by protein kinase Cdelta.
AuthorsSaharinen P, Ekman N, Sarvas K, Parker P, Alitalo K, Silvennoinen O
JournalBlood
PubMed ID9373245
Members of the hematopoietically expressed Tec tyrosine kinase family have an important role in hematopoietic signal transduction, as exemplified by the crucial role of Btk for B-cell differentiation and activation. Although a variety of cell surface receptors have been found to activate Tec tyrosine kinases, the specific signaling pathways and ... More
Heparin-binding protein (CAP37) is internalized in monocytes and increases LPS-induced monocyte activation.
AuthorsHeinzelmann M, Mercer-Jones MA, Flodgaard H, Miller FN
JournalJ Immunol
PubMed ID9605157
Previous studies have shown that the neutrophil-derived heparin-binding protein (HBP), also known as CAP37 or azurocidin, potentiates the LPS- induced release of proinflammatory cytokines (TNF-alpha, IL-1, and IL- 6) from isolated human monocytes. To date, the mechanisms by which HBP enhances LPS-induced monocyte activation have not been elucidated, and it ... More
Characterization of molecular interactions between intercellular adhesion molecule-1 and leukocyte function- associated antigen-1.
AuthorsWoska JR Jr, Morelock MM, Jeanfavre DD, Bormann BJ
JournalJ Immunol
PubMed ID8648112
The ability of soluble ICAM-1 (sICAM-1) to inhibit ICAM-1/LFA-1 adhesion events has been reported previously by numerous investigators. sICAM-1 has been demonstrated to inhibit various in vitro assays at concentrations ranging from 2 nM to greater than 40 microM. Given the hypothesis that circulating ICAM-1 modulates immune functions, the ability ... More
Sox9 and p300 cooperatively regulate chromatin-mediated transcription.
AuthorsFurumatsu T, Tsuda M, Yoshida K, Taniguchi N, Ito T, Hashimoto M, Ito T, Asahara H,
JournalJ Biol Chem
PubMed ID16109717
Chromatin structure is a fundamental component of gene regulation, expression, and cellular differentiation. We have previously reported that the multifunctional coactivator p300 is a member of the Sox9-related transcriptional apparatus and activates Sox9 (Sry-type high-mobility-group box 9)-dependent transcription during chondrogenesis. However, the mechanism of synergy between Sox9 and p300 in ... More
Asp-225 and glu-375 in autocatalytic attachment of the prosthetic heme group of lactoperoxidase.
Authors Colas Christophe; Kuo Jane M; Ortiz de Montellano Paul R;
JournalJ Biol Chem
PubMed ID11756449
The heme in lactoperoxidase is attached to the protein by ester bonds between the heme 1- and 5-methyl groups and Glu-375 and Asp-275, respectively. To investigate the cross-linking process, we have examined the D225E, E375D, and D225E/E375D mutants of bovine lactoperoxidase. The heme in the E375D mutant is only partially ... More
Interactions of CBL with BCR-ABL and CRKL in BCR-ABL-transformed myeloid cells.
AuthorsBhat A, Kolibaba K, Oda T, Ohno-Jones S, Heaney C, Druker BJ
JournalJ Biol Chem
PubMed ID9195915
The Philadelphia chromosome, detected in virtually all cases of chronic myelogenous leukemia (CML), is formed by a reciprocal translocation between chromosomes 9 and 22 that fuses BCR-encoded sequences upstream of exon 2 of c-ABL. The BCR-ABL fusion creates a gene whose protein product, p210BCR-ABL, has been implicated as the cause ... More
Comparison of Cak1p-like cyclin-dependent kinase-activating kinases.
Authors Tsakraklides Vasiliki; Solomon Mark J;
JournalJ Biol Chem
PubMed ID12084729
Cyclin-dependent kinases (cdks) coordinate progression through the eukaryotic cell cycle and require phosphorylation by a cdk-activating kinase (CAK) for full activity. In most eukaryotes Cdk7 is the catalytic subunit of a heterotrimeric CAK (Cdk7-cyclin H-Mat1) that is also involved in transcription as part of the transcription factor IIH complex. The ... More
Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor.
Authors Liedtke Carole M; Yun C H Chris; Kyle Nicole; Wang Dandan;
JournalJ Biol Chem
PubMed ID11956211
Protein kinase C (PKC) regulation of cystic fibrosis transmembrane regulator (CFTR) chloride function has been demonstrated in several cell lines, including Calu-3 cells that express native, wild-type CFTR. We demonstrated previously that PKC epsilon was required for cAMP-dependent CFTR function. The goal of this study was to determine whether PKC ... More
Mutations Targeted to a Predicted Helix in the Extreme Carboxyl-terminal Region of the Human Estrogen Receptor-alpha Alter Its Response to Estradiol and 4-Hydroxytamoxifen.
The human estrogen receptor-alpha, a member of the nuclear receptor superfamily, is a ligand-regulated transcriptional modulator. Because comparatively little is known about the extreme carboxyl-terminal region of the estrogen receptor (F domain), we used secondary structure prediction to design mutations that delete the F domain (S554stop), disrupt a possible turn ... More
Molecular cloning and characterization of a novel UDP-GlcNAc:GalNAc-peptide beta1,3-N-acetylglucosaminyltransferase (beta 3Gn-T6), an enzyme synthesizing the core 3 structure of O-glycans.
The core 3 structure of the O-glycan, GlcNAcbeta1-3GalNAcalpha1-serine/threonine, an important precursor in the biosynthesis of mucin-type glycoproteins, is synthesized by UDP-N-acetylglucosamine:GalNAc-peptide beta1,3-N- acetylglucosaminyltransferase (beta3Gn-T; core 3 synthase). The core 3 structure is restricted in its occurrence to mucins from specific tissues such as the stomach, small intestine, and colon. A ... More
Functional conservation of subfamilies of putative UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferases in Drosophila, Caenorhabditis elegans, and mammals. One subfamily composed of l(2)35Aa is essentail in Drosophila.
Authors Schwientek Tilo; Bennett Eric P; Flores Carlos; Thacker John; Hollmann Martin; Reis Celso A; Behrens Jane; Mandel Ulla; Keck Birgit; Schäfer Mireille A; Haselmann Kim; Zubarev Roman; Roepstorff Peter; Burchell Joy M; Taylor-Papadimitriou Joyce; Hollingsworth Michael A; Clausen Henrik;
JournalJ Biol Chem
PubMed ID11925450
The completed fruit fly genome was found to contain up to 15 putative UDP-N-acetyl-alpha-d-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-transferase) genes. Phylogenetic analysis of the putative catalytic domains of the large GalNAc-transferase enzyme families of Drosophila melanogaster (13 available), Caenorhabditis elegans (9 genes), and mammals (12 genes) indicated that distinct subfamilies of orthologous genes ... More