Osteopontin Human ELISA Kit

Catalog number: BMS2066

Invitrogen™  Related applications: Protein Assays and Analysis

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Description

The human Osteopontin ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Osteopontin.

Osteopontin (OPN) is is a highly phosphorylated sialoprotein that is a prominent component of the mineralized extracellular matrices of bones and teeth. The protein is highly phosphorylated and glycosylated. The nascent protein has a size of 33 kDa; after posttranslational modifications its molecular weight increases to 44 kDa. A non phosphorylated form of OPN is produced by normal rat kidney cells. It is also found in urine, where it inhibits kidney stone formation.

Additionally OPN is expressed by various tissues, such as bone, teeth, endometrial and epithelial tissues, and also macrophages, different types of tumors, T cells and smooth muscle cells. OPN is able to influence bone homeostasis by promoting differentiation of osteoclasts or by enhancing osteoclast activity. OPN protects against apoptosis and induces survival and proliferation in several cell types. OPN is also associated with tumor genesis.

Because of its ability to interact with multiple cell surface receptors, OPN plays a major role in many physiological and pathological pathways including wound healing, tumor genesis, inflammation, bone turnover and immune responses. Therefore the manipulation of plasma OPN levels is useful in the treatment of cancer metastasis, osteoporosis and autoimmune diseases, such as rheumatoid arthritis.

Conjugate
Biotin

Suitable Sample Types
cell culture supernatant, serum, plasma (EDTA, citrate, heparin)

Sample Volume
20 µL

Reported Application
ELISA

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Range: 0.47-30 ng/mL
Assay Sensitivity: 0.26 ng/mL
Detection Method: Colorimetric
Detector Antibody Conjugate: Biotin
For Use With (Equipment): Microplate Reader
Former Identifiers: Platinum, eBioscience
Gene Aliases: BNSP, BSPI, ETA-1, OPN
Gene ID: 6696
Gene Name: secreted phosphoprotein 1
Gene Symbol: SPP1
Immunoassay Kit Format: Coated ELISA Kit
Interassay CV: 6.1%
Intraassay CV: 6.7%
Label or Dye: HRP (Horseradish Peroxidase)
Product Size: 96 tests
Protein Name: Osteopontin
Sample Type (Specific): Cell Culture Supernatants, Plasma (EDTA, Citrate, Heparin), Serum
Sample Volume: 20 µL
Target (Kit Named): Osteopontin
Target Species (Named): Human
Target Species (Validated): Human
UniProt ID: P10451

Contents & storage

Aluminium pouch(es) with a Microplate coated with monoclonal antibody to human Osteopontin
Biotin-Conjugate anti-human Osteopontin polyclonal antibody
Streptavidin-HRP
Human Osteopontin Standard lyophilized, 60 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

Documents

Manuals & protocols