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          • Primary Antibodies ›
          • PAK1/2/3 Antibodies

          Bioss

          Phospho-PAK1/2/3 (Thr423) Polyclonal Antibody

          View all (15) PAK1/2/3 antibodies

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          Cite Phospho-PAK1/2/3 (Thr423) Polyclonal Antibody

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          Phospho-PAK1/2/3 (Thr423) Polyclonal Antibody

          Product Details

          BS-1652R

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:500
          -

          Immunohistochemistry (Frozen) (IHC (F))

          1:100-1:500
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          1:500-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          KLH conjugated synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of (Thr423).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein A

          Storage buffer

          0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA

          Contains

          0.02% ProClin 300

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          In mammals, there are several identified isoforms of p21-Activated Protein Kinases or PAKs: alpha-PAK (also known as PAK-1) and beta-PAK (also known as PAK-3) are mostly brain-specific, while gamma-PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated wit X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: activated protein kinase alpha; ADRB2; adriamycin resistance-associated; Alpha-PAK; aPAK; Beta-PAK; BPAK; CDC42/RAC effector kinase PAK-A; CDC42/RAC effector kinase PAK-B; CDKN1A; CLAP; EC 2.7.11.1; gamma pak; Gamma-PAK; hPAK3; kinase PAK1; Oligophrenin-3; p21 (CDKN1A)-activated kinase 1; p21 (CDKN1A)-activated kinase 2; p21 (CDKN1A)-activated kinase 3; p21 protein (Cdc42/Rac)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 3; p21-activated kinase 1; p21-activated kinase 2; p21-activated kinase 3; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 3 (yeast Ste20-related); p58; p65-PAK; p68-PAK; PAK 1; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK-3; PAK-a; PAK-B; PAK-y; PAK65; protein kinase; Protein kinase MUK2; S6/H4 kinase; Serine/threonine-protein kinase PAK 1; Serine/threonine-protein kinase PAK 2; Serine/threonine-protein kinase PAK 3; STE20 homolog, yeast; unnamed protein product; yPAK

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          Gene Aliases: 5330420P17Rik; A130002K10Rik; AI836325; alpha-PAK; ARA; AW045634; beta-PAK; bPAK; D16Ertd269e; gamma-PAK; IDDMSSD; KNO2; mKIAA4182; MRX30; MRX47; OPHN3; p65-PAK; PAK-1; PAK-2; PAK-3; PAK1; PAK2; PAK3; PAK3beta; PAK65; Pak65alpha; Pak65beta; Paka; PAKalpha; Pakb; PAKgamma; Stk4; XLID30

          View more View less

          UniProt ID: (Human) Q13153, (Human) Q13177, (Human) O75914, (Rat) P35465, (Mouse) Q8CIN4, (Rat) Q64303, (Mouse) Q61036, (Rat) Q62829

          View more View less

          Entrez Gene ID: (Human) 5058, (Human) 5062, (Human) 5063, (Mouse) 18479, (Rat) 29431, (Mouse) 224105, (Rat) 29432, (Mouse) 18481, (Rat) 29433

          View more View less

          Function(s)
          nucleotide binding catalytic activity protein kinase activity protein serine/threonine kinase activity protein binding collagen binding ATP binding kinase activity transferase activity protein kinase binding small GTPase binding identical protein binding gamma-tubulin binding protein serine kinase activity protein tyrosine kinase activator activity cadherin binding MAP kinase kinase activity SH3 domain binding metal ion binding transferase activity, transferring phosphorus-containing groups Rac GTPase binding MAP kinase kinase kinase activity receptor signaling protein serine/threonine kinase activity Rho GTPase binding GTPase binding non-receptor serine/threonine protein kinase
          Process(es)
          MAPK cascade response to hypoxia stimulatory C-type lectin receptor signaling pathway chromatin remodeling exocytosis apoptotic process cellular response to DNA damage stimulus transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cell proliferation cellular response to starvation positive regulation of fibroblast migration cell migration cerebellum development establishment of cell polarity actin cytoskeleton organization positive regulation of cell migration positive regulation of microtubule polymerization cellular response to insulin stimulus regulation of actin cytoskeleton organization positive regulation of intracellular estrogen receptor signaling pathway intracellular signal transduction Fc-gamma receptor signaling pathway involved in phagocytosis wound healing regulation of MAPK cascade positive regulation of axon extension positive regulation of insulin receptor signaling pathway hepatocyte growth factor receptor signaling pathway ephrin receptor signaling pathway branching morphogenesis of an epithelial tube neuron projection morphogenesis regulation of axonogenesis positive regulation of stress fiber assembly negative regulation of cell proliferation involved in contact inhibition negative regulation of cell growth involved in cardiac muscle cell development positive regulation of microtubule nucleation positive regulation of protein targeting to membrane regulation of actin filament organization protein localization to cytoplasmic stress granule positive regulation of vascular smooth muscle cell proliferation positive regulation of vascular associated smooth muscle cell migration cardiac muscle hypertrophy protein phosphorylation negative regulation of protein kinase activity signal transduction adherens junction assembly negative regulation of apoptotic process protein autophosphorylation vascular endothelial growth factor receptor signaling pathway regulation of cytoskeleton organization negative regulation of stress fiber assembly protein maturation dendritic spine development bicellular tight junction assembly cellular response to transforming growth factor beta stimulus execution phase of apoptosis protein localization to cell-cell junction positive regulation of execution phase of apoptosis positive regulation of extrinsic apoptotic signaling pathway axonogenesis dendrite development regulation of actin filament polymerization synapse organization dendritic spine morphogenesis regulation of postsynapse organization positive regulation of protein phosphorylation Rho protein signal transduction regulation of mitotic cell cycle neuromuscular junction development metabolic process response to organic substance regulation of gene expression phosphorylation amygdala development signal transduction by protein phosphorylation stress-activated protein kinase signaling cascade actin cytoskeleton reorganization activation of protein kinase activity positive regulation of peptidyl-serine phosphorylation regulation of apoptotic process receptor clustering positive regulation of JUN kinase activity gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission cellular response to organic cyclic compound observational learning regulation of long-term synaptic potentiation activation of MAPKK activity mitotic cell cycle small GTPase mediated signal transduction peptidyl-serine phosphorylation regulation of growth positive regulation of peptidyl-tyrosine phosphorylation positive regulation of protein tyrosine kinase activity negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis activation of MAPK activity multicellular organism development regulation of neuron projection development positive regulation of neuron apoptotic process positive regulation of dendritic spine morphogenesis positive regulation of DNA biosynthetic process
          It has to be done as per old AB suggested Products section.

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