One Shot™ PIR1 Chemically Competent E. coli
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One Shot&trade; PIR1 Chemically Competent <i>E. coli</i>
Invitrogen™

One Shot™ PIR1 Chemically Competent E. coli

One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replicationRead more
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Catalog NumberQuantity
C10101011 x 50 μL/tube
Catalog number C101010
Price (USD)
358.00
Special offer
Online exclusive
Ends: 21-Jul-2025
392.00
Save 34.00 (9%)
Each
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Quantity:
11 x 50 μL/tube
Price (USD)
358.00
Special offer
Online exclusive
Ends: 21-Jul-2025
392.00
Save 34.00 (9%)
Each
Add to cart

One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system). The pir gene encodes the replication protein π, which is required to replicate and maintain plasmids containing the R6Kγ origin. The PIR1 strain contains a mutant allele of the pir gene that maintains the donor vector construct at ∼250 copies per cell. PIR1 cells are competent and can achieve high transformation efficiency of >1 x 109 cfu/μg supercoiled plasmid.

R6Kγ allows tight control and selection and has been used in various applications including genome engineering, mutagenesis studies, bacterial artificial chromosome (BAC) construction, high-capacity extrachromosomal vector systems, and suicide plasmid construction.

One Shot PIR1 Chemically Competent E. coli cells offer:
pir gene to maintain vectors that contain the R6Kγ ori at ∼250 copies per cell
• Transformation efficiencies of >1 x 109 cfu/μg
endA1 mutation enables increased plasmid DNA yield and quantity
recA1 mutation reduce occurrence of unwanted recombination in cloned DNA
hsdR for efficient transformation of unmethylated DNA from PCR applications

Convenient and efficient format 
PIR1 Chemically Competent E. coli cells are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation, reducing freeze-thaw cycles and money wasted on unused cells.

Genotype
F∆lac169 rpoS(Am) robA1 creC510 hsdR514 endA recA1 uidA(∆MluI)::pir-116
Genetic marker descriptions

Find the strain and format that fit your needs
Multiple DH strains are available in chemically competent and electrocompetent cell formats.
We offer PIR2 strain that carries wild-type pir gene and maintain vectors at ∼15 copies per cell.
MultiShot formats are available for high throughput applications.
Explore bacterial growth media formats.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibiotic Resistance BacterialNo
Blue/White ScreeningNo
Cloning Methylated DNANo
Contains F' EpisomeNo
High-throughput CompatibilityLow
Improves Plasmid QualityYes (endA1)
Preparing Unmethylated DNANo
Product LineOne Shot™
Product TypeChemically Competent Cells
Quantity11 x 50 μL/tube
Reduces RecombinationYes (recA1)
Shipping ConditionDry Ice
T1 Phage - Resistant (tonA)No
Transformation Efficiency LevelHigh Efficiency (>1 x 109 cfu/μg)
FormatTube
SpeciesE. coli
Unit SizeEach
Contents & Storage
• One Shot PIR1 Chemically Competent E. coli (11 x 50 μL)
Store Competent Cells at –80°C.

• pUC19 plasmid (50 μL at 10 pg/μL)
Store pUC19 plasmid at –20°C.

• S.O.C. Medium
Store S.O.C. Medium at 4°C or room temperature.

Frequently asked questions (FAQs)

What is the strain of E.coli used in One Shot PIR1 Chemically Competent E. coli (Cat. No. C101010)?

The strain of E. coli used is K12.

Find additional tips, troubleshooting help, and resources within our Cloning Support Center.