Invitrogen™

One Shot™ PIR1 Chemically Competent E. coli

Catalog number:  C101010

Related applications:

Transformation

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Description

The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA strands. The end result is a fusion of the donor and acceptor vectors forming a single, functional expression vector.
PIR1 competent cells are for cloning and maintenance of your donor vector (i.e. pUniV5/His-TOPO®) construct (or other vector containing the R6K-gamma origin). It contains a mutant allele of the pir gene that maintains the donor vector construct at ~250 copies per cell.
For Research Use Only. Not for use in diagnostic procedures.

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Specifications

Bacterial or Yeast Strain: PIR1
Blue/White Screening: No
Cloning Methylated DNA: No
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Lacks F' Episome
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Product Line: One Shot™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^9
Format: One Shot
Improves Plasmid Quality: Yes
Species: E. coli

Contents & storage

One Shot® PIR1 Chemically Competent E. colicontains ten transformations of chemically competent cells in One Shot® format. Store at -80°C.