Invitrogen™

One Shot™ PIR1 Chemically Competent E. coli

Catalog number: C101010
Invitrogen™

One Shot™ PIR1 Chemically Competent E. coli

Catalog number: C101010
Catalog Number
C101010
Unit Size
11 x 50 µL/tube
Price (USD)
Price: 326.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C10101011 x 50 µL/tube
Price: 326.00
Your Price:
-
Product Overview
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Additional Information
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The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA strands. The end result is a fusion of the donor and acceptor vectors forming a single, functional expression vector.
PIR1 competent cells are for cloning and maintenance of your donor vector (i.e. pUniV5/His-TOPO™) construct (or other vector containing the R6K-gamma origin). It contains a mutant allele of the pir gene that maintains the donor vector construct at ∼250 copies per cell.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Competent Cell
Contains F' Episome
Lacks F' Episome
Efficiency
>1x10^9
Cell Type
Chemically Competent
Improves Plasmid Quality
Yes
Cloning Methylated DNA
No
Bacterial or Yeast Strain
PIR1
Blue/White Screening
No
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
T1 Phage - Resistant (tonA)
No
Species
E. coli
Format
One Shot
Product Line
One Shot™

Contents & Storage

One Shot™ PIR1 Chemically Competent E. colicontains ten transformations of chemically competent cells in One Shot™ format. Store at -80°C.