One Shot® PIR1 Chemically Competent E. coli

Catalog number: C101010

Invitrogen™  Related applications: Transformation

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA strands. The end result is a fusion of the donor and acceptor vectors forming a single, functional expression vector.
PIR1 competent cells are for cloning and maintenance of your donor vector (i.e. pUniV5/His-TOPO®) construct (or other vector containing the R6K? origin). It contains a mutant allele of the pir gene that maintains the donor vector construct at ~250 copies per cell.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Bacterial or Yeast Strain: PIR1
Blue-White Screening: No
Cloning Methylated DNA: No
Competent Cell Type: Chemically Competent
F' Episome (to make ssDNA): Lacks F' Episome
Format: One Shot
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: Yes
Product Size: 10 reactions
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
Species: E. coli
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^9

Contents & storage

One Shot® PIR1 Chemically Competent E. colicontains ten transformations of chemically competent cells in One Shot® format. Store at -80°C.

Documents

Manuals & protocols

Recommended products