Invitrogen™
One Shot™ PIR1 Chemically Competent E. coli
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Description
The Echo™ Cloning System is a two-vector system that utilizes the Cre-lox site-specific recombination system of bacteriophage P1 (1,2) for rapid cloning into multiple vectors. Your gene of interest is cloned into one of the specially designed donor vectors which can then be recombined with as many different Echo™ acceptor vectors as desired. The donor and acceptor vectors are placed in a buffer that contains Cre recombinase for a 25-minute, in vitro recombination reaction. Cre binds to the lox site present on each vector, brings them together, then cleaves and covalently reattaches the DNA strands. The end result is a fusion of the donor and acceptor vectors forming a single, functional expression vector.PIR1 competent cells are for cloning and maintenance of your donor vector (i.e. pUniV5/His-TOPO®) construct (or other vector containing the R6K-gamma origin). It contains a mutant allele of the pir gene that maintains the donor vector construct at ~250 copies per cell.
For Research Use Only. Not for use in diagnostic procedures.
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Specifications
| Bacterial or Yeast Strain: | PIR1 |
|---|---|
| Blue-White Screening: | No |
| Cloning Methylated DNA: | No |
| Competent Cell Type: | Chemically Competent |
| F' Episome (to make ssDNA): | Lacks F' Episome |
| Format: | One Shot |
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Improves Plasmid Quality: | Yes |
| Product Line: | One Shot® |
| Product Size: | 10 reactions |
| Propagating ccdB Vectors: | Not for ccdB vector propagation |
| Reduces Recombination: | Yes |
| Species: | E. coli |
| T1 Phage - Resistant (tonA): | No |
| Transformation Efficiency: | > 1x10^9 |
Contents & storage
One Shot® PIR1 Chemically Competent E. colicontains ten transformations of chemically competent cells in One Shot® format. Store at -80°C.
