Click-iT™ RNA Alexa Fluor™ 594 Imaging Kit

Click-iT™ RNA Alexa Fluor™ 594 Imaging Kit - FAQs

View additional product information for Click-iT™ RNA Alexa Fluor™ 594 Imaging Kit - FAQs (C10330)

3 product FAQs found

Can the components of the Click-iT Plus EdU Alexa Fluor Imaging Kits be used for labeling samples for flow cytometry?

Other than the EdU (Component A), DMSO (Component C), and Click-iT EdU buffer additive (Component F or G), all other components in the respective kits are not interchangeable. The types of reagents and amounts provided per kit were optimized either for imaging or flow cytometry. Using the imaging reagents may result in an excessive level of signal for flow cytometry detection and using the flow cytometry reagents may result in sub-optimal signal for imaging.

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Can any type of cell incorporate EU?

No. The cell must possess a pyrimidine salvage pathway; without this pathway, EU does not become phosphorylated to allow incorporation during RNA synthesis.

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What is the approximate fluorescence excitation/emission maxima for Alexa Fluor 594 azide?

The fluorescence excitation/emission maxima is 590/615 nm.

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