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Click-iT® RNA Alexa Fluor® 594 Imaging Kit This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: C10330

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Labeling Chemistry | Nucleic Acid Labeling & Oligo Synthesis

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Description

The Click-iT® RNA Alexa Fluor® 594 Imaging Kit enables detection of global RNA transcription temporally and spatially in cells and tissues (PNAS (2008) 105:15779-84).The ability to detect newly synthesized RNA or changes in RNA levels resulting from disease, environmental damage or drug treatments is an important aspect of toxicological profiling. Utilizing an alkyne-modified nucleoside, 5-ethynyl uridine (EU), and powerful click chemistry, newly synthesized RNA can be detected without the use of radioactivity or antibodies with a simple, two-step procedure. In step one, the alkyne-containing nucleoside is fed to cells or animals and actively incorporated into nascent RNA. The small size of the tag enables efficient incorporation of the modified nucleoside into RNA, but not into DNA. Detection utilizes the chemoselective ligation or “click" reaction between and azide and an alkyne where the modified RNA is detected with a corresponding azide-containing dye. With its dimunitive “footprint", the Click-iT® detection molecule can easily penetrate complex samples and leaves open the possibility of multiplex analyses with other probes, including antibodies for the detection of RNA-interactive proteins for deeper biological insights.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Red
Format: Slide(s)
Label or Dye: Alexa Fluor™ 594
Product Size: 1 kit
Emission Class: Visible
Detection Method: Fluorescent
Excitation Class: Visible
For Use With (Equipment): Fluorescence Microscope, Confocal Microscope
Excitation⁄Emission (nm): 590⁄617
Green Features: Less hazardous
Shipping Condition: Room Temperature

Contents & storage

The kit contains sufficient material for 25, 18 X 18 coverslips. Store at 2-6°C, dessicate and protect from light.

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