Click-iT™ Nascent RNA Capture Kit, for gene expression analysis - FAQs

View additional product information for Click-iT™ Nascent RNA Capture Kit, for gene expression analysis - FAQs (C10365)

2 product FAQs found

What are the main characteristics of a Click-iT reaction?

Click reactions have several general characteristics: the reaction is efficient, no extreme temperatures or solvents are required, the reaction is complete within 30 minutes, the components of the reaction are bio-inert, and perhaps most importantly, no side reactions occur-the label and detection tags react selectively and specifically with one another. This final point is a key advantage of this powerful detection technique; it is possible to apply click chemistry-labeled molecules to complex biological samples and detect them with unprecedented sensitivity due to the extremely low background of the reaction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the Click-iT Nascent RNA Capture Kit, for gene expression analysis (Cat. No. C10365) be used in downstream next-generation sequencing (NGS) applications?

Yes, cDNA produced at the end of the Click-iT Nascent RNA Capture Kit, for gene expression analysis (Cat. No. C10365) workflow (step 6.8 on page 11 of the Manual) can be used for next-generation sequencing (NGS) application.

Below are some references of the downstream NGS application using this kit:

Palozola, K. C., Donahue, G., & Zaret, K. S. (2021). EU-RNA-seq for in vivo labeling and high throughput sequencing of nascent transcripts. STAR protocols, 2(3), 100651.

Lu, X. M., Batugedara, G., Lee, M., Prudhomme, J., Bunnik, E. M., & Le Roch, K. G. (2017). Nascent RNA sequencing reveals mechanisms of gene regulation in the human malaria parasite Plasmodium falciparum. Nucleic acids research, 45(13), 7825-7840.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.