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CellROX® Reagent Variety Pack, for oxidative stress detection

Catalog number: C10448

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Flow Cytometry

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Description

The CellROX® Variety Pack contains one vial (50 µL) each of CellROX® Green Reagent, CellROX® Orange Reagent, and CellROX® Deep Red Reagent. These reagents are novel fluorogenic probes for measuring cellular oxidative stress in live cells. These cell-permeant dyes exhibit bright fluorescence upon oxidation by reactive oxygen species (ROS). (See table below for details of each reagent.)

The CellROX® reagents are:

• Optimized for detection of oxidative stress in live cells and may be added directly to serum-containing growth media
• Provided as a stable, ready-to-use DMSO solutions with simple protocols, compatible with standard workflows in fluorescence microscopy
• Compatible with a variety of detection platforms, including traditional fluorescence microscopy, high content screening (HCS), and flow cytometry

Oxidative stress results from an imbalance between the production of reactive oxygen species (ROS) and the ability of cells to scavenge them. ROS play an important role in the progression of several diseases, including inflammation, atherosclerosis, aging, and age-related degenerative disorders.

 Catalog # Absorption/Emission (Color) Live cell compatible? Formaldehyde fixable?Detergent resistant? Platforms
CellROX® Deep Red ReagentC10422 644/665 nm (Deep Red) Yes YesNo Imaging, HCS, HTS, flow cytometry, Attune™
CellROX® Orange ReagentC10443 545/565 nm (Orange) Yes NoNo Imaging, HCS, flow cytometry, Tali™
CellROX® Green ReagentC10444 485/520 nm (Green) Yes YesYes Imaging, HCS, HTS, FLOW, Tali™, FLoid™, Attune™


For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Indicator Type: Free Radical Sensor
Form: Frozen
Color: Orange, Green, Far-Red
Solubility: DMSO (Dimethylsulfoxide)
Product Size: 1 kit
Detection Method: Fluorescent
For Use With (Equipment): Fluorescence Microscope, HTS Reader, Flow Cytometer, High Content Instrument, Floid® Cell Imaging System, Attune® Acoustic Focusing Cytometer
Shipping Condition: Wet Ice

Contents & storage

Store at ≤-20°C

Protect from light

Desiccate when possible

Documents

Manuals & protocols

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