CellROX™ Reagent Variety Pack, for oxidative stress detection
CellROX™ Reagent Variety Pack, for oxidative stress detection
Invitrogen™

CellROX™ Reagent Variety Pack, for oxidative stress detection

El paquete variado CellROX™ contiene un vial (50 μl) de cada reactivo: CellROX™ Green, CellROX™ Orange y CellROX™ Deep Red.Más información
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Número de catálogoCantidad
C104481 kit
Número de catálogo C10448
Precio (MXN)
-
Cantidad:
1 kit
El paquete variado CellROX™ contiene un vial (50 μl) de cada reactivo: CellROX™ Green, CellROX™ Orange y CellROX™ Deep Red. Estos reactivos son nuevas sondas fluorogénicas para medir el estrés oxidativo celular en células vivas. Estos colorantes que penetran en las células presentan una fluorescencia brillante tras la oxidación por especies reactivas de oxígeno (ROS). (Consulte la tabla siguiente para obtener más información sobre cada reactivo.)

Los reactivos CellROX™:

• Están optimizados para la detección del estrés oxidativo en células vivas. Se puede añadir directamente a medios de crecimiento que contienen suero
• Se suministran como soluciones de DMSO estables y listas para su uso con un sencillo protocolo compatible con los flujos de trabajo estándar de la microscopía de fluorescencia
• Son compatibles con una variedad de plataformas de detección, incluidas la microscopía de fluorescencia tradicional, el cribado masivo de información (HCS) y la citometría de flujo

El estrés oxidativo resulta de un desequilibrio entre la producción de especies reactivas de oxígeno (ROS) y la capacidad de las células para eliminarlas. Las ROS juegan un papel importante en la progresión de varias enfermedades, incluyendo la inflamación, la aterosclerosis, el envejecimiento y los trastornos degenerativos relacionados con la edad.

 Nº de cat. Absorción/emisión (color) Compatible con células vivas? Se puede fijar con formaldehído?Resistente a detergentes? Plataformas
Reactivo CellROX™ Deep RedC10422 644/665 nm (rojo intenso) No Adquisición de imágenes, HCS, HTS, citometría de flujo, Attune™
Reactivo CellROX™ OrangeC10443 545/565 nm (naranja) NoNo Adquisición de imágenes, HCS, citometría de flujo, Tali™
Reactivo CellROX™ GreenC10444 485/520 nm (verde) Adquisición de imágenes, HCS, HTS, FLUJO, Tali™, FLoid™, Attune™


Para uso exclusivo en investigación. No diseñado para uso terapéutico o de diagnóstico en animales o humanos.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorNaranja, rojo lejano, verde
FormatoCongelado
Cantidad1 kit
Unit SizeEach

Preguntas frecuentes

What dyes can I use to detect reactive oxygen species (ROS) in my bacteria?

Many dyes that are used on mammalian cells have also been shown to be useful in bacterial cells. For example, CellROX Deep Red Reagent has been shown to work in B. subtilis (see Reference: http://www-brs.ub.ruhr-uni-bochum.de/netahtml/HSS/Diss/RaatschenNadja/diss.pdf). If you are interested in a particular dye, but are not sure if it will work on your bacteria, literature searches are the best way to check to see if it has been tested. If not, then it may be worth testing yourself.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (15)

Citations & References
Abstract
Mechanisms of programmed cell death signaling in hair cells and support cells post-electrode insertion trauma.
Authors:Eshraghi AA, Lang DM, Roell J, Van De Water TR, Garnham C, Rodrigues H, Guardiola M, Gupta C, Mittal J,
Journal:
PubMed ID:25761716
'Programmed cell death (PCD) initially starts in the support cells (SCs) after electrode insertion trauma (EIT), followed by PCD in hair cells (HCs). Activation of caspase-3 was observed only in SCs. Protecting both SCs and HCs with selective otoprotective drugs at an early stage post implantation may help to preserve ... More
Dynamics of sub-lethal effects of nano-CuO on the microalga Chlamydomonas reinhardtii during short-term exposure.
Authors:von Moos N, Maillard L, Slaveykova VI,
Journal:
PubMed ID:25731685
Though nano-CuO has been classified as toxic toward aquatic microorganisms and its use in various applications is expected to increase in near future, its ecotoxicity is currently poorly understood. The aim of this study was to investigate the mechanisms of nano-CuO toxicity based on the paradigm of oxidative stress, the ... More
Development of poly(ethylene glycol) hydrogels for salivary gland tissue engineering applications.
Authors:Shubin AD, Felong T, Graunke D, Ovitt CE, Benoit D,
Journal:
PubMed ID:25762214
Over 40,000 patients are diagnosed with head and neck cancers annually in the US with the vast majority receiving radiation therapy. Salivary glands are irreparably damaged by radiation therapy resulting in xerostomia, which severely affects patient quality of life. Cell-based therapies have shown some promise in mouse models of radiation-induced ... More
Oxidative stress triggers cytokinesis failure in hepatocytes upon isolation.
Authors:Tormos AM, Taléns-Visconti R, Bonora-Centelles A, Pérez S, Sastre J,
Journal:
PubMed ID:25744598
Primary hepatocytes are highly differentiated cells and proliferatively quiescent. However, the stress produced during liver digestion seems to activate cell cycle entry by proliferative/dedifferentiation programs that still remain unclear. The aim of this work was to assess whether the oxidative stress associated with hepatocyte isolation affects cell cycle and particularly ... More
DNA Damage: A Sensible Mediator of the Differentiation Decision in Hematopoietic Stem Cells and in Leukemia.
Authors:Weiss CN, Ito K,
Journal:
PubMed ID:25789504
In the adult, the source of functionally diverse, mature blood cells are hematopoietic stem cells, a rare population of quiescent cells that reside in the bone marrow niche. Like stem cells in other tissues, hematopoietic stem cells are defined by their ability to self-renew, in order to maintain the stem ... More