Mechanisms of programmed cell death signaling in hair cells and support cells post-electrode insertion trauma.
AuthorsEshraghi AA, Lang DM, Roell J, Van De Water TR, Garnham C, Rodrigues H, Guardiola M, Gupta C, Mittal J,
Journal
PubMed ID25761716
'Programmed cell death (PCD) initially starts in the support cells (SCs) after electrode insertion trauma (EIT), followed by PCD in hair cells (HCs). Activation of caspase-3 was observed only in SCs. Protecting both SCs and HCs with selective otoprotective drugs at an early stage post implantation may help to preserve ... More
Dynamics of sub-lethal effects of nano-CuO on the microalga Chlamydomonas reinhardtii during short-term exposure.
Authorsvon Moos N, Maillard L, Slaveykova VI,
Journal
PubMed ID25731685
Though nano-CuO has been classified as toxic toward aquatic microorganisms and its use in various applications is expected to increase in near future, its ecotoxicity is currently poorly understood. The aim of this study was to investigate the mechanisms of nano-CuO toxicity based on the paradigm of oxidative stress, the ... More
Development of poly(ethylene glycol) hydrogels for salivary gland tissue engineering applications.
Over 40,000 patients are diagnosed with head and neck cancers annually in the US with the vast majority receiving radiation therapy. Salivary glands are irreparably damaged by radiation therapy resulting in xerostomia, which severely affects patient quality of life. Cell-based therapies have shown some promise in mouse models of radiation-induced ... More
Oxidative stress triggers cytokinesis failure in hepatocytes upon isolation.
AuthorsTormos AM, Taléns-Visconti R, Bonora-Centelles A, Pérez S, Sastre J,
Journal
PubMed ID25744598
Primary hepatocytes are highly differentiated cells and proliferatively quiescent. However, the stress produced during liver digestion seems to activate cell cycle entry by proliferative/dedifferentiation programs that still remain unclear. The aim of this work was to assess whether the oxidative stress associated with hepatocyte isolation affects cell cycle and particularly ... More
DNA Damage: A Sensible Mediator of the Differentiation Decision in Hematopoietic Stem Cells and in Leukemia.
AuthorsWeiss CN, Ito K,
Journal
PubMed ID25789504
In the adult, the source of functionally diverse, mature blood cells are hematopoietic stem cells, a rare population of quiescent cells that reside in the bone marrow niche. Like stem cells in other tissues, hematopoietic stem cells are defined by their ability to self-renew, in order to maintain the stem ... More
Highly specific in vivo gene delivery for p53-mediated apoptosis and genetic photodynamic therapies of tumour.
AuthorsTseng SJ, Liao ZX, Kao SH, Zeng YF, Huang KY, Li HJ, Yang CL, Deng YF, Huang CF, Yang SC, Yang PC, Kempson IM,
Journal
PubMed ID25739372
Anticancer therapies are often compromised by nonspecific effects and challenged by tumour environments' inherent physicochemical and biological characteristics. Often, therapeutic effect can be increased by addressing multiple parameters simultaneously. Here we report on exploiting extravasation due to inherent vascular leakiness for the delivery of a pH-sensitive polymer carrier. Tumours' acidic ... More
A tryptophanol-derived oxazolopiperidone lactam is cytotoxic against tumors via inhibition of p53 interaction with murine double minute proteins.
AuthorsSoares J, Raimundo L, Pereira NA, Dos Santos DJ, Pérez M, Queiroz G, Leão M, Santos MM, Saraiva L,
Journal
PubMed ID25814188
Inactivation of the p53 tumor suppressor protein by interaction with murine double minute (MDM) proteins, MDM2 and MDMX, is a common event in human tumors expressing wild-type p53. In these tumors, the simultaneous inhibition of these interactions with MDMs, for a full p53 reactivation, represents a promising anticancer strategy. Herein, ... More
Identification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase as a novel autophagy regulator by high content shRNA screening.
AuthorsStrohecker AM, Joshi S, Possemato R, Abraham RT, Sabatini DM, White E,
Journal
PubMed ID25772235
Deregulation of autophagy has been linked to multiple degenerative diseases and cancer, thus the identification of novel autophagy regulators for potential therapeutic intervention is important. To meet this need, we developed a high content image-based short hairpin RNA screen monitoring levels of the autophagy substrate p62/SQSTM1. We identified 186 genes ... More
Iron Homeostasis and Pulmonary Hypertension: Iron Deficiency Leads to Pulmonary Vascular Remodeling in the Rat.
AuthorsCotroneo E, Ashek A, Wang L, Wharton J, Dubois OD, Bozorgi SS, Busbridge M, Alavian KN, Wilkins MR, Zhao L,
Journal
PubMed ID25767292
Iron deficiency without anaemia is prevalent in patients with idiopathic pulmonary arterial hypertension and associated with reduced exercise capacity and survival. We hypothesised that iron deficiency is involved in the pathogenesis of pulmonary hypertension and iron replacement is a possible therapeutic strategy. Rats were fed an iron-deficient diet (IDD, 7mg/kg) ... More
Effect of uraemia on endothelial cell damage is mediated by the integrin linked kinase pathway.
AuthorsGarcía-Jérez A, Luengo A, Carracedo J, Ramírez-Chamond R, Rodriguez-Puyol D, Rodriguez-Puyol M, Calleros L,
Journal
PubMed ID25398526
Patients with chronic kidney disease have a higher risk of developing cardiovascular diseases than the general population. Their vascular endothelium is dysfunctional, among other things, because it is permanently exposed to uraemic toxins, several of which have poor clearance by conventional dialysis. Recent studies have demonstrated the important role of ... More
Biologically Active Metabolites Produced by the Basidiomycete Quambalaria cyanescens.
AuthorsStodulková E, Císarová I, Kolarík M, Chudícková M, Novák P, Man P, Kuzma M, Pavlu B, Cerný J, Flieger M,
Journal
PubMed ID25723150
Four strains of the fungus Quambalaria cyanescens (Basidiomycota: Microstromatales), were used for the determination of secondary metabolites production and their antimicrobial and biological activities. A new naphthoquinone named quambalarine A, (S)-(+)-3-(5-ethyl-tetrahydrofuran-2-yliden)-5,7,8-trihydroxy-2-oxo-1,4-naphthoquinone (1), together with two known naphthoquinones, 3-hexanoyl-2,5,7,8-tetrahydroxy-1,4-naphthoquinone (named here as quambalarine B, 2) and mompain, 2,5,7,8-tetrahydroxy-1,4-naphthoquinone (3) were isolated. ... More
TGF-ß1 induces epithelial-to-mesenchymal transition via inhibiting mitochondrial functions in A549 cells.
AuthorsZhang J, Zhang W, Zhang T, Zhou Q, Liu J, Liu Y, Kong D, Yu W, Liu R, Hai C
JournalFree Radic Res
PubMed ID30203703
'Epithelial-to-mesenchymal transition (EMT) is critical to the progression of several disease processes including carcinoma metastasis and organ fibrosis. Recent studies show that reactive oxygen species (ROS) and mitochondrial dysfunction have been associated with EMT. However, the role of mitochondria in the EMT process remains to be elucidated. Through the induction ... More
Cisplatin induces autophagy to enhance hepatitis B virus replication via activation of ROS/JNK and inhibition of the Akt/mTOR pathway.
AuthorsChen X, Hu Y, Zhang W, Chen K, Hu J, Li X, Liang L, Cai X, Hu J, Wang K, Huang A, Tang N
JournalFree Radic Biol Med
PubMed ID30550853
'Chronic hepatitis B virus (HBV) infection remains a serious global health concern. Cisplatin is a chemotherapeutic agent commonly used to treat various cancers. However, HBV-infected patients receiving chemotherapy are at risk of HBV reactivation via unknown mechanisms, which we aimed to elucidate in this study. We found that autophagy plays ... More
Macrophage LC3-associated phagocytosis is an immune defense against Streptococcus pneumoniae that diminishes with host aging.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID33376222
Autoimmunity gene IRGM suppresses cGAS-STING and RIG-I-MAVS signaling to control interferon response.