A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part III: Reagents for Actin, Tubulin, Cellular Membranes, and Whole Cell and Cytoplasm.
AuthorsKilgore JA, Dolman NJ, Davidson MW,
Journal
PubMed ID24510770
'Non-antibody commercial fluorescent reagents for imaging of cytoskeletal structures have been limited primarily to tubulin and actin, with the main factor in choice based mainly on whether cells are live or fixed and permeabilized. A wider range of options exist for cell membrane dyes, and the choice of reagent primarily ... More
Stress fibers stabilize the position of intranuclear DNA through mechanical connection with the nucleus in vascular smooth muscle cells.
AuthorsNagayama K, Yahiro Y, Matsumoto T,
JournalFEBS Lett
PubMed ID22094165
Actin stress fibers (SFs) running across the top surface of the nucleus in vascular smooth muscle cells were dissected using laser nano-dissection technique to release its pretension, and the dynamic behavior of SFs, nucleus, and intranuclear DNA were investigated. SFs shortened across the top surface of the nuclei after their ... More
Role of c-Met/phosphatidylinositol 3-kinase (PI3k)/Akt signaling in hepatocyte growth factor (HGF)-mediated lamellipodia formation, reactive oxygen species (ROS) generation, and motility of lung endothelial cells.
Hepatocyte growth factor (HGF) mediated signaling promotes cell proliferation and migration in a variety of cell types and plays a key role in tumorigenesis. As cell migration is important to angiogenesis, we characterized HGF-mediated effects on the formation of lamellipodia, a pre-requisite for migration using human lung microvascular endothelial cells ... More
Viscoelastic cell mechanics and actin remodelling are dependent on the rate of applied pressure.
AuthorsPravincumar P, Bader DL, Knight MM,
JournalPLoS One
PubMed ID22984454
Living cells are subjected to external and internal mechanical stresses. The effects of these stresses on the deformation and subsequent biological response of the cells remains unclear. This study tested the hypothesis that the rate at which pressure (or stress) is applied influence the viscoelastic properties of the cell associated ... More
Baculovirus-mediated gene transfer into mammalian cells.
AuthorsBoyce FM, Bucher NL,
JournalProc Natl Acad Sci U S A
PubMed ID8637876
This paper describes the use of the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) as a vector for gene delivery into mammalian cells. A modified AcMNPV virus was prepared that carried the Escherichia coli lacZ reporter gene under control of the Rous sarcoma virus promoter and mammalian RNA processing ... More
BacMam technology and its application to drug discovery.
AuthorsAmes RS, Kost TA, Condreay JP,
JournalExpert Opin Drug Discov
PubMed ID23488908
The recombinant baculovirus/insect cell system was firmly established as a leading method for recombinant protein production when a new potential use for these viruses was revealed in 1995. It was reported that engineered recombinant baculoviruses could deliver functional expression cassettes to mammalian cell types; a system which has come to ... More
Mapping the Mechanome-A Protocol for Simultaneous Live Imaging and Quantitative Analysis of Cell Mechanoadaptation and Ingression.
Authors
JournalBio Protoc
PubMed ID33654934
Wnt5A/Ryk signaling critically affects barrier function in human vascular endothelial cells.
Authors
JournalCell Adh Migr
PubMed ID27159116
Selective Labeling of Individual Neurons in Dense Cultured Networks With Nanoparticle-Enhanced Photoporation.
Authors
JournalFront Cell Neurosci
PubMed ID29651235
Modification of BRCA1-associated breast cancer risk by HMMR overexpression.
Authors
JournalNat Commun
PubMed ID35393420
IL-4 Causes Hyperpermeability of Vascular Endothelial Cells through Wnt5A Signaling.
Authors
JournalPLoS One
PubMed ID27214384
Fluorogenic Probes for Multicolor Imaging in Living Cells.
Authors
JournalJ Am Chem Soc
PubMed ID27420907
A Role for the Chaperone Complex BAG3-HSPB8 in Actin Dynamics, Spindle Orientation and Proper Chromosome Segregation during Mitosis.
Authors
JournalPLoS Genet
PubMed ID26496431
Fluorogenic probes for live-cell imaging of the cytoskeleton.