CellLight™ Actin-RFP, BacMam 2.0
Description
CellLight® Actin-RFP, BacMam 2.0, provides an easy way to label actin with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools
This ready-to-use construct is transfected into cells using BacMam 2.0 technology and expresses RFP fused to human actin with almost no cytotoxic effects. Study the dynamic action of actin filaments in live cells and multiplex with other fluorescent proteins or organic dyes.
Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.
CellLight® Technology is:
• Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
• Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
• Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
• Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling
BacMam Technology
CellLight® Actin-RFP, BacMam 2.0, is a fusion construct of human actin and TagRFP, providing accurate and specific targeting to cellular actin-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.
Figures
CellLight® Reagent Workflow
Human epidermal keratinocytes, neonatal, (HEKn) were co-transduced with CellLight® Actin-RFP and CellLight® Tubulin-GFP and imaged on a Zeiss LSM confocal microscope after over night incubation.
Human epidermal keratinocytes, neonatal, (HEKn) were co-transduced with CellLight® Actin-RFP and CellLight® Late Endosomes-GFP and imaged on a Zeizz LSM confocal microscope after over night incubation.
Human epidermal keratinocytes, neonatal, (HEKn) were co-transduced with CellLight® Actin-RFP and CellLight® Late Endosomes-GFP and imaged on a Zeiss LSM confocal microscope after over night incubation.
Human epidermal keratinocytes, neonatal, (HEKn) were co-transduced with CellLight® Actin-RFP and CellLight® Talin-GFP and imaged on a Zeiss LSM confocal microscope after over night incubation.
Human epidermal keratinocytes, neonatal, (HEKn) were co-transduced with CellLight® Actin-RFP and CellLight® Talin-GFP and imaged on a Zeiss LSM confocal microscope after over night incubation.
Human epidermal keratinocytes, neonatal, (HEKn) were co-transduced with CellLight® Actin-RFP and CellLight® Talin-GFP and imaged on a Zeiss LSM confocal microscope after over night incubation.
Human epidermal keratinocytes, neonatal, (HEKn) were co-transduced with CellLight® Actin-RFP and CellLight® Late Endosomes-GFP and imaged on a Zeiss LSM confocal microscope after over night incubation.
Human epidermal keratinocytes, neonatal, (HEKn) were transduced with CellLight® Actin-RFP and imaged on a Zeiss LSM confocal microscope after over night incubation.
Principle of BacMam Reagents
Specifications
| Emission: | Visible |
|---|---|
| Excitation: | Visible |
| Excitation/Emission: | 555⁄584 |
| Form: | Liquid |
| Label or Dye: | RFP (TagRFP) |
| Product Line: | CellLight®, Molecular Probes® |
| Shipping Condition: | Wet Ice |
| Sub Cellular Localization: | Actin |
| Target Function: | Cell Structure |
| Detection Method: | Fluorescent |
| For Use With (Equipment): | Fluorescence Microscope, Flow Cytometer, Fluorescent Imager |
| Quantity: | 1 vial(s) |
| Label Type: | Fluorescent Protein |
| Color: | Red-Orange |
