CellLight fluorescent proteins provide an easy way to label specific structures in live cells. Simply add the ready-to-use constructs to your cells, incubate overnight, and you’re ready to image in the morning. These constructs express fluorescent fusion proteins targeted to specific intracellular structures. The fluorescent protein is introduced using a simple transfection step, using the BacMam technology, that doesn’t require molecular biology techniques to carry out—it works like a cell stain.
|Excitation wavelength range
|Histones (histone 2B)
|BacMam 2.0 transduction control
|Null virus (control)
Using the BacMam 2.0-based CellLight fluorescent fusion proteins to express genes is as simple as it is efficient:
Cells can be transduced by mixing cells with the CellLight fluorescent fusion protein immediately prior to plating. Alternatively, adherent cells can be transduced by addition of virus to cells already in dish or on plate. Typically, optimal transduction is obtained at 70% confluence and with a 20:1 BacMam particle to cell ratio.
BacMam technology provides you with an efficient transduction system so that you can create relevant cell models to run your assays. Use BacMam technology to label organelles with targeted fluorescent proteins, express drug targets such as ion channels or receptors, or monitor cellular processes with fluorescent protein indicators. You can also create your own custom constructs using Invitrogen ViraPower BacMam Expression System.
For Research Use Only. Not for use in diagnostic procedures.