Microscopic image of cells labeled with green mitochondria fusion protein, red talin cytoskeletal fusion protein, and blue nucleus stain

CellLight fluorescent proteins provide an easy way to label specific structures in live cells. Simply add the ready-to-use constructs to your cells, incubate overnight, and you’re ready to image in the morning. These constructs express fluorescent fusion proteins targeted to specific intracellular structures. The fluorescent protein is introduced using a simple transfection step, using the BacMam technology, that doesn’t require molecular biology techniques to carry out—it works like a cell stain.

See all CellLight fluorescent protein products

Advantages of labeling with CellLight fluorescent proteins

  • Specific intracellular targets ideal for your experimental needs
  • Simple add-incubate-image protocol for each cell structure
  • Multiplex with other CellLight proteins, antibodies, or organic dyes

CellLight fluorescent protein selection guide

Excitation wavelength range488-510555-584435-485
Size1 mL1 mL1 mL
Endoplasmic reticulumC10590C10591
Early endosomesC10586C10587
Late endosomesC10588C10589
Histones (histone 2B)C10594C10595
Plasma membraneC10607C10608C10606
BacMam 2.0 transduction controlB10383
Null virus (control)C10615

CellLight workflow

Schematic of adding CellLight fluorescent proteins to your cells

Using the BacMam 2.0-based CellLight fluorescent fusion proteins to express genes is as simple as it is efficient:

  1. Add virus to cells in full medium.
  2. Incubate overnight.
  3. Image or freeze for future use.

Cells can be transduced by mixing cells with the CellLight fluorescent fusion protein immediately prior to plating. Alternatively, adherent cells can be transduced by addition of virus to cells already in dish or on plate. Typically, optimal transduction is obtained at 70% confluence and with a 20:1 BacMam particle to cell ratio.

BacMam delivery technology

BacMam technology provides you with an efficient transduction system so that you can create relevant cell models to run your assays. Use BacMam technology to label organelles with targeted fluorescent proteins, express drug targets such as ion channels or receptors, or monitor cellular processes with fluorescent protein indicators. You can also create your own custom constructs using Invitrogen ViraPower BacMam Expression System.

Guidelines for using CellLight fluorescent fusion proteins

When to use CellLight fluorescent fusion proteins:

  • To target organelles or intracellular structures in live cells with minimal toxicity or chemical disruption
  • To identify functions that require higher resolution
  • To multiplex with other CellLight fluorescent proteins or organic dyes to follow the behavior of individual cells, and analyze in live cells or after fixation
  • To label mitochondria irrespective of membrane potential and lysosomes irrespective of pH

When NOT to use CellLight fluorescent fusion proteins:

  • During cell population studies or automated imaging/counting because CellLight fluorescent fusion proteins rarely result in 100% transfection rates (Transfection rates and conditions may differ by cell type as a result an extensive reference list on their uses is provided)

CellLight image gallery

  • Mitochondria and cytoskeleton staining in live HeLa cells
  • Cytoskeleton staining in live HEKn cells
  • Oxidative stress detection in human osteosarcoma (U2OS) cells
  • Peroxisome and golgi staining in live HEKn cells
  • Cytoskeleton staining and autophagy detection in live U2OS cells
  • Monitoring autophagy, tubulin, and mitochondria in live U2OS cells
  • Cytoskeleton staining in live HEKn cells
  • Nucleus and mitochondria staining in live HeLa cells
  • Cytoskeleton staining in live HEKn cells

CellLight reagent videos

Real-time visualization of neuronal growth cone dynamics

Time-lapse movie of U2OS cell division

Stylesheet for Classic Wide Template adjustments

For Research Use Only. Not for use in diagnostic procedures.