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CellLight® Late Endosomes-GFP, BacMam 2.0

Catalog number: C10588

Molecular Probes™  Related applications: Cell Structure | Cellular Imaging

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Description

CellLight® Late Endosomes-GFP, BacMam 2.0, provides an easy way to label late endosomes with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to Rab7a. You can observe late endosomes-GFP behavior in live cells independently of organelle pH and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology

CellLight® Late Endosomes-GFP, BacMam 2.0, is a fusion construct of Rab7a and emGFP, providing accurate and specific targeting to cellular Late Endosomes-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.

Specifications

Form: Liquid
Color: Green
Quantity: 1 vial(s)
Label or Dye: GFP (EmGFP)
Product Size: 1 mL
Emission Class: Visible
Detection Method: Fluorescent
Excitation Class: Visible
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Fluorescent Imager
Sub-Cellular Localization: Endosomes
Excitation⁄Emission (nm): 488⁄510
Shipping Condition: Wet Ice

Contents & storage

2-6°C, protect from light. Do not freeze.

Documents

Manuals & protocols