A Rab11a-enriched subapical membrane compartment regulates a cytoskeleton-dependent transcytotic pathway in secretory epithelial cells of the lacrimal gland.
AuthorsXu S, Edman M, Kothawala MS, Sun G, Chiang L, Mircheff A, Zhu L, Okamoto C, Hamm-Alvarez S,
JournalJ Cell Sci
PubMed ID21984810
'Despite observations that the lacrimal gland has been identified as the principal source of dimeric immunoglobulin A (dIgA) in tears, the mechanism used by lacrimal gland acinar cells (LGACs) to transcytose dIgA produced by interstitial plasma cells is not well-characterized. This study identifies a transcytotic pathway in LGACs regulated by ... More
Visualizing the endocytosis of phenylephrine in living cells by quantum dot-based tracking.
AuthorsMa J, Wu L, Hou Z, Song Y, Wang L, Jiang W,
Journal
PubMed ID24855959
'To study the intracellular receptor-drug transportation, a fluorescent probe consisting of phenylephrine-polyethylene glycol-quantum dots conjugate was employed to track endocytosis process of phenylephrine in living cells. This type of movement was studied by continuously filming fluorescent images in the same cell. We also calculated the movement parameters, and divided the ... More
Particles on the move: intracellular trafficking and asymmetric mitotic partitioning of nanoporous polymer particles.
AuthorsYan Y, Lai ZW, Goode RJ, Cui J, Bacic T, Kamphuis MM, Nice EC, Caruso F,
Journal
PubMed ID23713907
'Nanoporous polymer particles (NPPs) prepared by mesoporous silica templating show promise as a new class of versatile drug/gene delivery vehicles owning to their high payload capacity, functionality, and responsiveness. Understanding the cellular dynamics of such particles, including uptake, intracellular trafficking, and distribution, is an important requirement for their development as ... More
Intracellular dynamics and fate of polystyrene nanoparticles in A549 Lung epithelial cells monitored by image (cross-) correlation spectroscopy and single particle tracking.
AuthorsDeville S, Penjweini R, Smisdom N, Notelaers K, Nelissen I, Hooyberghs J, Ameloot M,
Journal
PubMed ID26164626
Novel insights in nanoparticle (NP) uptake routes of cells, their intracellular trafficking and subcellular targeting can be obtained through the investigation of their temporal and spatial behavior. In this work, we present the application of image (cross-) correlation spectroscopy (IC(C)S) and single particle tracking (SPT) to monitor the intracellular dynamics ... More
Probing site-exclusive binding of aqueous QDs and their organelle-dependent dynamics in live cells by single molecule spectroscopy.
AuthorsDong C, Chowdhury B, Irudayaraj J,
JournalAnalyst
PubMed ID23493749
Understanding the biophysical and chemical interactions of nanoprobes and their fate upon entering live cells is critical for developing fundamental insights related to intracellular diagnostics, drug delivery and targeting. In this article we report herein a single molecule analysis procedure to quantitate site-specific exclusive membrane binding of N-acetyl-L-cysteine (NAC)-capped cadmium ... More
Overcoming endosomal barrier by amphotericin B-loaded dual pH-responsive PDMA-b-PDPA micelleplexes for siRNA delivery.
AuthorsYu H, Zou Y, Wang Y, Huang X, Huang G, Sumer BD, Boothman DA, Gao J,
JournalACS Nano
PubMed ID22011045
The endosomal barrier is a major bottleneck for the effective intracellular delivery of siRNA by nonviral nanocarriers. Here, we report a novel amphotericin B (AmB)-loaded, dual pH-responsive micelleplex platform for siRNA delivery. Micelles were self-assembled from poly(2-(dimethylamino)ethyl methacrylate)-block-poly(2-(diisopropylamino)ethyl methacrylate) (PDMA-b-PDPA) diblock copolymers. At pH 7.4, AmB was loaded into the ... More
Baculovirus-mediated gene transfer into mammalian cells.
AuthorsBoyce FM, Bucher NL,
JournalProc Natl Acad Sci U S A
PubMed ID8637876
This paper describes the use of the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) as a vector for gene delivery into mammalian cells. A modified AcMNPV virus was prepared that carried the Escherichia coli lacZ reporter gene under control of the Rous sarcoma virus promoter and mammalian RNA processing ... More
BacMam technology and its application to drug discovery.
AuthorsAmes RS, Kost TA, Condreay JP,
JournalExpert Opin Drug Discov
PubMed ID23488908
The recombinant baculovirus/insect cell system was firmly established as a leading method for recombinant protein production when a new potential use for these viruses was revealed in 1995. It was reported that engineered recombinant baculoviruses could deliver functional expression cassettes to mammalian cell types; a system which has come to ... More
Targeted Delivery of C/EBPα-saRNA by RNA Aptamers Shows Anti-tumor Effects in a Mouse Model of Advanced PDAC.
Authors
JournalMol Ther Nucleic Acids
PubMed ID31546149
A food-responsive switch modulates TFEB and autophagy, and determines susceptibility to coxsackievirus infection and pancreatitis.
Authors
JournalAutophagy
PubMed ID32019403
Targeted Delivery of Deoxycytidine Kinase to Her2-Positive Cells Enhances the Efficacy of the Nucleoside Analog Fludarabine.
AuthorsKoduvayur SP, Su Y, Kay BK, Lavie A,
JournalPLoS One
PubMed ID27280468
'Cytotoxic drugs, such as nucleoside analogs and toxins, commonly suffer from off-target effects. One approach to mitigate this problem is to deliver the cytotoxic drug selectively to the intended site. While for toxins this can be achieved by conjugating the cell-killing moiety to a targeting moiety, it is not an ... More
Immuno-potentiating pathway of HBsAg-HBIG immunogenic complex visualized.
AuthorsLiu H, Geng S, Wang B, Wu B, Xie X, Wang S, Zhong Y, Wang X, Qu D, Wen Y, Wang B,
JournalHum Vaccin Immunother
PubMed ID26618396
'Chronic viral hepatitis B (CHB) is a major global health problem. A therapeutic vaccine for CHB comprised of yeast-derived recombinant HBsAg-anti-HBs immunogenic complexes (YIC) has been devloped by us. A series of clinical trials has shown its therapeutic efficacy in decreasing HBV viral load and converting serum HBeAg-positive to anti-HBe-positive ... More
Spatiotemporally and Sequentially-Controlled Drug Release from Polymer Gatekeeper-Hollow Silica Nanoparticles.
Authors
JournalSci Rep
PubMed ID28436438
Structural Analysis of the Myo1c and Neph1 Complex Provides Insight into the Intracellular Movement of Neph1.
Authors
JournalMol Cell Biol
PubMed ID27044863
Endosome motility defects revealed at super-resolution in live cells using HIDE probes.
Authors
JournalNat Chem Biol
PubMed ID32094922
Pooled Screens Identify GPR108 and TM9SF2 as Host Cell Factors Critical for AAV Transduction.
Authors
JournalMol Ther Methods Clin Dev
PubMed ID32280726
Chemical Modulation of Endocytic Sorting Augments Adeno-associated Viral Transduction.
Authors
JournalJ Biol Chem
PubMed ID26527686
The epithelial polarity regulator LGALS9/galectin-9 induces fatal frustrated autophagy in KRAS mutant colon carcinoma that depends on elevated basal autophagic flux.
Authors
JournalAutophagy
PubMed ID26086204
Sulfisoxazole inhibits the secretion of small extracellular vesicles by targeting the endothelin receptor A.
Authors
JournalNat Commun
PubMed ID30918259
Transit and integration of extracellular mitochondria in human heart cells.
Authors
JournalSci Rep
PubMed ID29234096
Contribution of enhanced engagement of antigen presentation machinery to the clinical immunogenicity of a human interleukin (IL)-21 receptor-blocking therapeutic antibody.
AuthorsXue L, Hickling T, Song R, Nowak J, Rup B,
JournalClin Exp Immunol
PubMed ID26400440
Reliable risk assessment for biotherapeutics requires accurate evaluation of risk factors associated with immunogenicity. Immunogenicity risk assessment tools were developed and applied to investigate the immunogenicity of a fully human therapeutic monoclonal antibody, ATR-107 [anti-interleukin (IL)-21 receptor] that elicited anti-drug antibodies (ADA) in 76% of healthy subjects in a Phase ... More