Invitrogen™

CellLight™ Nucleus-GFP, BacMam 2.0

Référence: C10602
Invitrogen™

CellLight™ Nucleus-GFP, BacMam 2.0

Référence: C10602
Référence
C10602
Taille de l’unité
1 mL
Prix (EUR)
Prix 552,00
Votre prix
Disponibilité
***
Quantité
RéférenceTaille de l’unitéPrix (EUR)DisponibilitéQuantité
C106021 mL
Prix 552,00
Votre prix
***
Présentation du produit
Figures
Vidéos
Documentation
Foire aux questions
Citations et références
Informations supplémentaires
Recommandations
Recommandations
CellLight™ Nucleus-GFP, BacMam 2.0, provides an easy way to label nuclei with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight™ fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses GFP fused to the SV40 nuclear localization sequence. You can observe nucleus-GFP behavior in live cells without the cellular toxicity associated with intercalators and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight™ constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight™ Technology is:
Fast and convenient: simply add CellLight™ reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight™ reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight™ Nucleus-GFP, BacMam 2.0, is a fusion construct of SV40 nuclear localization sequence and emGFP, providing accurate and specific targeting to cellular nucleus-GFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight™ reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.
For Research Use Only. Not for use in diagnostic procedures.

Spécifications

Couleur
Vert
Gamme de longueur d’onde d’excitation
488 / 510
Type de colorant
GFP (EmGFP)
À utiliser avec (équipement)
Microscope confocal, microscope à fluorescence
Quantité
1 flacon
Méthode de détection
Fluorescent
Émission
Visible
Forme
Liquide
Gamme de produits
CellLight™
Conditions d’expédition
Glace humide
Technique
Intensité de fluorescence
Type d’étiquette
Protéine fluorescente
Type de produit
Nucleus
SubCellular Localization
Nucleus

Contenu et stockage

Stocker à 2°C à 6°C, à l’abri de la lumière. Ne pas congeler.