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BacMam GFP Transduction Control (BacMam 2.0)

Catalog number: B10383

Molecular Probes™  Related applications: Cellular Imaging

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Description

The BacMam 2.0 Transduction Control lets you experience the power and convenience of enhanced BacMam technology, with the convenient readout of emerald GFP (emGFP); our Aequorea victoria emGFP is extremely bright and photo stable and this non-targeted form is ideal for testing transduction. Check out how well BacMam 2.0 works with GFP in your model before advancing to the more specialized reagents.

BacMam 2.0 provides several advantages over other gene transfer methods:

• Efficient: highly efficient (>90%) transduction of a wide range of mammalian cell lines, including primary cells, stem cells and neuronal cells
Fast and convenient: Simply add the BacMam 2.0 together with cells to your cover slip or microtiter plate, incubate overnight and assay – or store frozen, assay-ready cells for later use
Safe: non-replicating in mammalian cells, lack of observable cytopathic effect and biosafety level (BSL) 1 handling
Enables Flexiblity: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies and tightly control expression levels by just varying the dose

The BacMam technology is based on an insect virus (baculovirus) to help efficiently deliver and express genes in mammalian cells. The baculovirus has been modified to include an expression cassette for transgene expression in mammalian cells. BacMam 2.0 incorporate elements that help greatly enhance transduction efficiency and expression levels: a pseudotyped capsid protein for more efficient cell entry and genetic elements (enhanced CMV promoter and Woodchuck Post-transcriptional Regulatory Element), that boost expression levels. Baculoviruses do not replicate in mammalian cells and thus have an excellent safety profile and lack cytopathic effects on cells.

Cell types previously not compatible with the technology (neuronal cells, T-cells), or only poorly transduced (some stem cells, CHO) are now transduced quantitatively in a simple, one step process.

We offer a range of BacMam-based reagents, including Cellular Lights™ cell labeling reagents, Premo™ Biosensors, ion channel drug targets, pathway analysis kits, and more. To learn more about these products, please visit www.invitrogen.com ⁄bacmam.

Today the other BacMam 2.0-based reagent is our enabling Premo™ Autophagy Sensor for autophagy research in live cells.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Color: Green
Promoter: CMV
Quantity: 1 vial
Technique: Fluorescence Intensity
Label or Dye: GFP (EmGFP)
Product Size: 1 mL
Emission Class: Visible
Detection Method: Fluorescent
Excitation Class: Visible
For Use With (Equipment): Fluorescence Microscope, High Content Instrument, Flow Cytometer, Fluorometer, HTS Reader
Sub-Cellular Localization: Nucleus, Cytoplasm
Flow Cytometer Laser Lines: 488
Excitation⁄Emission (nm): 485⁄520
Shipping Condition: Wet Ice

Contents & storage

The BacMam GFP ttransduction control contains 1 ml of virus at a concentration of approximately 1 × 10^8 viral particles⁄ml.
Approximate fluorescence excitation⁄emission maxima for emGFP: 485⁄520 in nm.
The BacMam transduction control should be stored at 4°C, protected from light. When stored as directed, it is stable for 6 months. DO NOT FREEZE.

Documents

Manuals & protocols