CellLight™ Nucleus-RFP, BacMam 2.0

Catalog number:  C10603

Related applications:

Cell Structure

| Cell Tracing & Tracking

| Cellular Imaging

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CellLight® Nucleus-RFP, BacMam 2.0, provides an easy way to label nuclei with red fluorescent protein (RFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

Want to label other cell structures? Learn more about CellLight® fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses RFP fused to the SV40 nuclear localization sequence. You can observe nucleus-RFP behavior in live cells without the cellular toxicity associated with intercalators and label with multiple tracking or tracing dyes to image dynamic cellular processes.

Cells expressing CellLight® constructs can also be fixed with formaldehyde for multiplexed imaging using immunocytochemical techniques.

CellLight® Technology is:
Fast and convenient: simply add CellLight® reagent to your cells, incubate overnight, and image—or store frozen, assay-ready cells for later use
Highly efficient: up to 90% transduction of a wide range of mammalian cell lines, including primary cells, stem cells, and neurons
Flexible: co-transduce more than one BacMam reagent for multiplex experiments or co-localization studies; tightly control expression levels by simply varying the dose
Less toxic: CellLight® reagents are non-replicating in mammalian cells and are suitable for biosafety level (BSL) 1 handling

BacMam Technology
CellLight® Nucleus-RFP, BacMam 2.0, is a fusion construct of SV40 nuclear localization sequence and TagRFP, providing accurate and specific targeting to cellular nucleus-RFP. This fusion construct is packaged in the insect virus baculovirus, which does not replicate in human cells and is designated as safe to use with biosafety level (BSL) 1 in most laboratories. BacMam technology ensures that most mammalian cell types are transduced/transfected with high efficiency and minimal toxicity. This transient transfection can be detected after overnight incubation for up to five days—enough time to carry out most dynamic cellular analyses. Like any transfection/transduction technique, the BacMam method does not transfect/transduce all of the cells with equal efficiency, making it poorly suited to cellular population studies or automated imaging/counting. CellLight® reagents are ideal for experiments where cellular or subcellular co-locatization is required, or for cellular function studies that need special resolution.
For Research Use Only. Not for use in diagnostic procedures.


Emission: Visible
Excitation: Visible
Excitation/Emission: 555⁄584
Flow Cytometer Laser Lines: 532
Form: Liquid
Label or Dye: RFP (TagRFP)
Product Line: CellLight®
Shipping Condition: Wet Ice
Sub Cellular Localization: Nucleus
Technique: Fluorescence Intensity
Detection Method: Fluorescent
For Use With (Equipment): Confocal Microscope, Fluorescence Microscope
Quantity: 1 vial
Label Type: Fluorescent Protein
Color: Red-Orange, Orange

Contents & storage

Store at 2-6°C, protected from light. Do Not Freeze.