Targeted Polymersome Delivery of siRNA Induces Cell Death of Breast Cancer Cells Dependent upon Orai3 Protein Expression.
AuthorsPangburn TO, Georgiou K, Bates FS, Kokkoli E,
JournalLangmuir
PubMed ID22827285
Polymersomes, polymeric vesicles that self-assemble in aqueous solutions from block copolymers, have been avidly investigated in recent years as potential drug delivery agents. Past work has highlighted peptide-functionalized polymersomes as a highly promising targeted delivery system. However, few reports have investigated the ability of polymersomes to operate as gene delivery ... More
Identification and Characterization of Receptor-Specific Peptides for siRNA Delivery.
AuthorsRen Y, Hauert S, Lo JH, Bhatia SN,
JournalACS Nano
PubMed ID22909216
Tumor-targeted delivery of siRNA remains a major barrier in fully realizing the therapeutic potential of RNA interference. While cell-penetrating peptides (CPP) are promising siRNA carrier candidates, they are universal internalizers that lack cell-type specificity. Herein, we design and screen a library of tandem tumor-targeting and cell-penetrating peptides that condense siRNA ... More
Cell-permeable gomesin peptide promotes cell death by intracellular Ca(2+) overload.
AuthorsParedes-Gamero EJ, Casaes-Rodrigues RL, Moura GE, Domingues TM, Buri MV, Ferreira VH, Trindade ES, Moreno-Ortega AJ, Cano-Abad MF, Nader HB, Ferreira AT, Miranda A, Justo GZ, Tersariol IL,
JournalMol Pharm
PubMed ID22873645
In recent years, the antitumoral activity of antimicrobial peptides (AMPs) has been the goal of many research studies. Among AMPs, gomesin (Gm) displays antitumor activity by unknown mechanisms. Herein, we studied the cytotoxicity of Gm in the Chinese hamster ovary (CHO) cell line. Furthermore, we investigated the temporal ordering of ... More
A Rab11a-enriched subapical membrane compartment regulates a cytoskeleton-dependent transcytotic pathway in secretory epithelial cells of the lacrimal gland.
AuthorsXu S, Edman M, Kothawala MS, Sun G, Chiang L, Mircheff A, Zhu L, Okamoto C, Hamm-Alvarez S,
JournalJ Cell Sci
PubMed ID21984810
'Despite observations that the lacrimal gland has been identified as the principal source of dimeric immunoglobulin A (dIgA) in tears, the mechanism used by lacrimal gland acinar cells (LGACs) to transcytose dIgA produced by interstitial plasma cells is not well-characterized. This study identifies a transcytotic pathway in LGACs regulated by ... More
Deletion of lipoprotein PG0717 in Porphyromonas gingivalis W83 reduces gingipain activity and alters trafficking in and response by host cells.
AuthorsReyes L, Eiler-McManis E, Rodrigues PH, Chadda AS, Wallet SM, Bélanger M, Barrett AG, Alvarez S, Akin D, Dunn WA, Progulske-Fox A,
Journal
PubMed ID24069284
'P. gingivalis (Pg), a causative agent of chronic generalized periodontitis, has been implicated in promoting cardiovascular disease. Expression of lipoprotein gene PG0717 of Pg strain W83 was found to be transiently upregulated during invasion of human coronary artery endothelial cells (HCAEC), suggesting this protein may be involved in virulence. We ... More
Cell entry and trafficking of human adenovirus bound to blood factor X is determined by the fiber serotype and not hexon:heparan sulfate interaction.
AuthorsCorjon S, Gonzalez G, Henning P, Grichine A, Lindholm L, Boulanger P, Fender P, Hong SS,
JournalPLoS One
PubMed ID21637339
'Human adenovirus serotype 5 (HAdV5)-based vectors administered intravenously accumulate in the liver as the result of their direct binding to blood coagulation factor X (FX) and subsequent interaction of the FX-HAdV5 complex with heparan sulfate proteoglycan (HSPG) at the surface of liver cells. Intriguingly, the serotype 35 fiber-pseudotyped vector HAdV5F35 ... More
A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part III: Reagents for Actin, Tubulin, Cellular Membranes, and Whole Cell and Cytoplasm.
AuthorsKilgore JA, Dolman NJ, Davidson MW,
Journal
PubMed ID24510770
'Non-antibody commercial fluorescent reagents for imaging of cytoskeletal structures have been limited primarily to tubulin and actin, with the main factor in choice based mainly on whether cells are live or fixed and permeabilized. A wider range of options exist for cell membrane dyes, and the choice of reagent primarily ... More
Improving drug potency and efficacy by nanocarrier-mediated subcellular targeting.
AuthorsMurakami M, Cabral H, Matsumoto Y, Wu S, Kano MR, Yamori T, Nishiyama N, Kataoka K,
JournalSci Transl Med
PubMed ID21209412
'Nanocarrier-mediated drug targeting is an emerging strategy for cancer therapy and is being used, for example, with chemotherapeutic agents for ovarian cancer. Nanocarriers are selectively accumulated in tumors as a result of their enhanced permeability and retention of macromolecules, thereby enhancing the antitumor activity of the nanocarrier-associated drugs. We investigated ... More
Cellular delivery of polynucleotides by cationic cyclodextrin polyrotaxanes.
AuthorsDandekar P, Jain R, Keil M, Loretz B, Muijs L, Schneider M, Auerbach D, Jung G, Lehr CM, Wenz G,
JournalJ Control Release
PubMed ID22789529
Cationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water-soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA. Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The ... More
Cytokeratin 8 is an epithelial cell receptor for pet, a cytotoxic serine protease autotransporter of enterobacteriaceae.
AuthorsNava-Acosta R, Navarro-Garcia F,
Journal
PubMed ID24327340
ABSTRACT The group of proteins known as serine protease autotransporters of Enterobacteriaceae (SPATE) is a growing family of serine proteases secreted to the external milieu by the type V secretion system. Pet toxin and some other SPATE belong to the class 1 cytotoxic SPATE, which have comparable protease strength on ... More
AuthorsRankin CR, Hilgarth RS, Leoni G, Kwon M, Den Beste KA, Parkos CA, Nusrat A,
JournalJ Biol Chem
PubMed ID23558678
The gastrointestinal epithelium functions as an important barrier that separates luminal contents from the underlying tissue compartment and is vital in maintaining mucosal homeostasis. Mucosal wounds in inflammatory disorders compromise the critical epithelial barrier. In response to injury, intestinal epithelial cells (IECs) rapidly migrate to reseal wounds. We have previously ... More
Fluorescent peptide-PNA chimeras for imaging monoamine oxidase A mRNA in neuronal cells.
AuthorsSethi D, Chen CP, Jing RY, Thakur ML, Wickstrom E,
JournalBioconjug Chem
PubMed ID22239616
Monoamine oxidases (MAO) catalyze the oxidative deamination of many biogenic amines and are integral proteins found in the mitochondrial outer membrane. Changes in MAO-A levels are associated with depression, trait aggression, and addiction. Here we report the synthesis, characterization, and in vitro evaluation of novel fluorescent peptide-peptide nucleic acid (PNA) ... More
PEGylated liposomal doxorubicin targeted to a5ß1-expressing MDA-MB-231 breast cancer cells.
AuthorsShroff K, Kokkoli E,
JournalLangmuir
PubMed ID22268611
Targeting drugs selectively to cancer cells can potentially benefit cancer patients by avoiding side effects generally associated with several cancer therapies. One of the attractive approaches to direct the drug cargo to specific sites is to incorporate ligands at the surface of the delivery systems. Integrin a(5)ß(1) is overexpressed in ... More
Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells.
AuthorsHosoya S, Villena J, Shimazu T, Tohno M, Fujie H, Chiba E, Shimosato T, Aso H, Suda Y, Kawai Y, Saito T, Alvarez S, Ikegami S, Itoh H, Kitazawa H,
JournalVet Res
PubMed ID22046952
ABSTRACT: This study analyzed the functional expression of TLR3 in various gastrointestinal tissues from adult swine and shows that TLR3 is expressed preferentially in intestinal epithelial cells (IEC), CD172a+CD11R1high and CD4+ cells from ileal Peyer's patches. We characterized the inflammatory immune response triggered by TLR3 activation in a clonal porcine ... More
Spreading of neurodegenerative pathology via neuron-to-neuron transmission of ß-amyloid.
Alzheimer's disease (AD) is the major cause of dementia. During the development of AD, neurofibrillary tangles progress in a fixed pattern, starting in the transentorhinal cortex followed by the hippocampus and cortical areas. In contrast, the deposition of ß-amyloid (Aß) plaques, which are the other histological hallmark of AD, does ... More
Baculovirus-mediated gene transfer into mammalian cells.
AuthorsBoyce FM, Bucher NL,
JournalProc Natl Acad Sci U S A
PubMed ID8637876
This paper describes the use of the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) as a vector for gene delivery into mammalian cells. A modified AcMNPV virus was prepared that carried the Escherichia coli lacZ reporter gene under control of the Rous sarcoma virus promoter and mammalian RNA processing ... More
BacMam technology and its application to drug discovery.
AuthorsAmes RS, Kost TA, Condreay JP,
JournalExpert Opin Drug Discov
PubMed ID23488908
The recombinant baculovirus/insect cell system was firmly established as a leading method for recombinant protein production when a new potential use for these viruses was revealed in 1995. It was reported that engineered recombinant baculoviruses could deliver functional expression cassettes to mammalian cell types; a system which has come to ... More
IFITM3 directly engages and shuttles incoming virus particles to lysosomes.
AuthorsSpence JS, He R, Hoffmann HH, Das T, Thinon E, Rice CM, Peng T, Chandran K, Hang HC
JournalNat Chem Biol
PubMed ID30643282
'Interferon-induced transmembrane proteins (IFITMs 1, 2 and 3) have emerged as important innate immune effectors that prevent diverse virus infections in vertebrates. However, the cellular mechanisms and live-cell imaging of these small membrane proteins have been challenging to evaluate during viral entry of mammalian cells. Using CRISPR-Cas9-mediated IFITM-mutant cell lines, ... More
A food-responsive switch modulates TFEB and autophagy, and determines susceptibility to coxsackievirus infection and pancreatitis.
Authors
JournalAutophagy
PubMed ID32019403
The class II phosphoinositide 3-kinases PI3K-C2a and PI3K-C2ß differentially regulate clathrin-dependent pinocytosis in human vascular endothelial cells.
AuthorsAung KT, Yoshioka K, Aki S, Ishimaru K, Takuwa N, Takuwa Y
JournalJ Physiol Sci
PubMed ID30374841
'Pinocytosis is an important fundamental cellular process that is used by the cell to transport fluid and solutes. Phosphoinositide 3-kinases (PI3Ks) regulate a diverse array of dynamic membrane events. However, it is not well-understood which PI3K isoforms are involved in specific mechanisms of pinocytosis. We performed knockdown studies of endogenous ... More
LYST affects lysosome size and quantity, but not trafficking or degradation through autophagy or endocytosis.
Authors
JournalTraffic
PubMed ID25216107
Methyl-β-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK.
Authors
JournalAutophagy
PubMed ID28613987
Mechanisms of Silver Nanoparticle Uptake by Embryonic Zebrafish Cells.
Authors
JournalNanomaterials (Basel)
PubMed ID34685144
Interaction between TIM-1 and NPC1 Is Important for Cellular Entry of Ebola Virus.
Authors
JournalJ Virol
PubMed ID25855742
Endosomal dysfunction in iPSC-derived neural cells from Parkinson's disease patients with VPS35 D620N.
Authors
JournalMol Brain
PubMed ID33032646
Crossing the blood-brain-barrier with nanoligand drug carriers self-assembled from a phage display peptide.
Authors
JournalNat Commun
PubMed ID31604928
Transit and integration of extracellular mitochondria in human heart cells.
Authors
JournalSci Rep
PubMed ID29234096
Aberrant early endosome biogenesis mediates complement activation in the retinal pigment epithelium in models of macular degeneration.
AuthorsKaur G, Tan LX, Rathnasamy G, La Cunza N, Germer CJ, Toops KA, Fernandes M, Blenkinsop TA, Lakkaraju A
JournalProc Natl Acad Sci U S A
PubMed ID30126999
Abnormally enlarged early endosomes (EEs) are pathological features of neurodegenerative diseases, yet insight into the mechanisms and consequences of EE expansion remains elusive. Here, we report swollen apical EEs in the retinal pigment epithelium (RPE) of aged human donors and in the pigmented ... More
Targeted delivery of lysosomal enzymes to the endocytic compartment in human cells using engineered extracellular vesicles.
AuthorsDo MA, Levy D, Brown A, Marriott G, Lu B
JournalSci Rep
PubMed ID31754156
Targeted delivery of lysosomal enzymes to the endocytic compartment of human cells represents a transformative technology for treating a large family of lysosomal storage diseases (LSDs). Gaucher disease is one of the most common types of LSDs caused by mutations to the lysosomal ß-glucocerebrosidase (GBA). Here, we describe a genetic ... More