'Complications of microocclusions have been reported after intra-arterial delivery of mesenchymal stromal cells. Hence, quantification and efficient limitation of cell clumps in suspension before transplantation is important to reduce the risk. We used a flow cytometry-based pulse-width assay to assess the effects of different cell suspension concentrations (0.2-2.0 × 10(6)/mL), ... More
New flow cytometry approaches in equine andrology.
AuthorsPeña FJ, Ortega Ferrusola C, Martín Muñoz P
JournalTheriogenology
PubMed ID27160445
Flow cytometry is currently recognized as a robust tool for the evaluation of sperm quality and function. However, within equine reproduction, this technique has not reached the sophistication of other areas of biology and medicine. In recent years, more sophisticated flow cytometers have been introduced in andrology laboratories, and the ... More
Avenanthramide-C reduces the viability of MDA-MB-231 breast cancer cells through an apoptotic mechanism.
AuthorsHastings J, Kenealey J
JournalCancer Cell Int
PubMed ID29075150
Avenanthramides (AVN) are a relatively unstudied family of phytochemicals that could be novel chemotherapeutics. These compounds, found in oats, are non-toxic to healthy cells and have been shown to reduce viability of human colon and liver cancers in vitro. However, these studies do not elucidate a molecular mechanism for individual ... More
Comparison of
AuthorsOpydo-Chanek M, Sladowska K, Blicharski K, Mikeš J, Fedorocko P, Niemeyer U, Mazur L
JournalAnticancer Res
PubMed ID29061820
The oxazaphosphorines, ifosfamide and cyclophosphamide, represent a class of alkylating agents. The aim of the present in vitro study was to compare antileukemic activity of 4-hydroperoxyifosfamide (4-OOH-IF) and 4-hydroperoxycyclophosphamide (4-OOH-CP). ... More
Liposomal encapsulation of silver nanoparticles enhances cytotoxicity and causes induction of reactive oxygen species-independent apoptosis.
AuthorsYusuf A, Brophy A, Gorey B, Casey A
JournalJ Appl Toxicol
PubMed ID29181855
Silver nanoparticles (AgNP) are one of the most widely investigated metallic NPs due to their promising antibacterial activities. In recent years, AgNP research has shifted beyond antimicrobial use to potential applications in the medical arena. This shift coupled with the extensive commercial applications of AgNP will further increase human exposure ... More
(-)-Epigallocatechin-3-gallate, reduces corneal damage secondary from experimental grade II alkali burns in mice.
AuthorsGulias-Cañizo R, Lagunes-Guillén A, González-Robles A, Sánchez-Guzmán E, Castro-Muñozledo F
JournalBurns
PubMed ID30600126
Since recent reports have shown that (-)-Epigallocatechin-3-gallate (EGCG) could be used for treating proliferative and inflammatory disorders, we explored its use for the management of corneal chemical burns. ... More
Non-Thermal Plasma, as a New Physicochemical Source, to Induce Redox Imbalance and Subsequent Cell Death in Liver Cancer Cell Lines.
AuthorsSmolková B, Lunova M, Lynnyk A, Uzhytchak M, Churpita O, Jirsa M, Kubinová Š, Lunov O, Dejneka A
JournalCell Physiol Biochem
PubMed ID30790509
Alteration of cancer cell redox status has been recognized as a promising therapeutic implication. In recent years, the emerged field of non-thermal plasma (NTP) has shown considerable promise in various biomedical applications, including cancer therapy. However, understanding the molecular mechanisms procuring cellular responses remains incomplete. Thus, the aim of this ... More