Invitrogen™

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Catalog number:  C10740

 Related applications:

Cell Viability, Proliferation & Function

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Description

The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent, as well as SYTOX™ AADvanced™ Dead Cell Stain.

View a selection guide for all apoptosis assays for flow cytometry >

• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Easy identification—clearly identify live, dead, and apoptotic cell populations
• Quick analysis—washing and fixation is not required
• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasers

CellEvent Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD, a caspase 3/7 recognition sequence) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and cleave the peptide. Cleavage of the peptide allows binding of DNA by the reagent, thereby labeling the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ~502/530 nm. When used together with the SYTOX AADvanced Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.

Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis. For more information, see Apoptosis Reagents in Flow Cytometry.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Fluorescent
For Use With (Equipment): Attune® Acoustic Focusing Cytometer, Flow Cytometer
Format: Kit
Product Line: CellEvent™
Product Size: 20 assays
Shipping Condition: Wet Ice

Contents & storage

• CellEvent Caspase 3/7 Green Detection Reagent
• SYTOX AADvanced Dead Cell Stain
• DMSO 

Documents

Manuals & protocols