Invitrogen™

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Catalog number:  C10740

Related applications:

Cell Viability, Proliferation & Function

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Description

The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent, as well as SYTOX™ AADvanced™ Dead Cell Stain.

• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Easy identification—clearly identify live, dead, and apoptotic cell populations
• Quick analysis—washing and fixation is not required
• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasers

CellEvent™ Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ~511/533 nm. When used together with the SYTOX™ AADvanced™ Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.

Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Line: CellEvent™
Shipping Condition: Wet Ice
Detection Method: Fluorescent
For Use With (Equipment): Attune™ Acoustic Focusing Cytometer, Flow Cytometer
Quantity: 20 Assays
Format: Kit

Contents & storage

• CellEvent Caspase 3/7 Green Detection Reagent
• SYTOX AADvanced Dead Cell Stain
• DMSO