Invitrogen™

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Catalog number: C10427
Have Questions?
Invitrogen™

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

Catalog number: C10427
Have Questions?
Catalog Number
C10427
Unit Size
100 assays
Price (USD)
Availability
-
Quantity
Catalog Number
Unit Size
20 assays
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
C10427100 assays
-
C1074020 assays
-
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The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent, as well as SYTOX™ AADvanced™ Dead Cell Stain.

• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Easy identification—clearly identify live, dead, and apoptotic cell populations
• Quick analysis—washing and fixation is not required
• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasers

CellEvent™ Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ∼511/533 nm. When used together with the SYTOX™ AADvanced™ Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.

Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity
100 Assays
Format
Kit
Product Type
Caspase Assay Kit
Flow Cytometer Laser Lines
488
Excitation/Emission
SYTOX AADvanced: 546/647
CellEvent Caspase 3/7 Green Detection Reagent: 511/533
Shipping Condition
Wet Ice
Solubility
DMSO (Dimethylsulfoxide)
Product Line
CellEvent™
Detection Method
Fluorescence
For Use With (Application)
Flow Cytometry
For Use With (Equipment)
Attune™ Acoustic Focusing Cytometer, Flow Cytometer
Label or Dye
CellEvent™ Caspase 3/7 Green Detection Reagent, SYTOX AADvanced dead cell stain
Label Type
Other Label(s) or Dye(s)

Contents & Storage

Contains 1 vial CellEvent™ Caspase 3/7 Green Detection Reagent, 1 vial SYTOX AADvanced dead cell stain, and 1 vial of DMSO.

Store all vials in the kit upright, at or below -20deg;C, desiccated, and protected from light.

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