Use ProLong® Live Antifade Reagent for brightest fluorescent images with live cells
Invitrogen™

5-(and-6)-Carboxy SNARF™-1

Catalog number:  C1270

Related applications:

Cell Viability, Proliferation & Function

Back to top

Description

The cell-impermeant pH indicator, carboxy SNARF®-1 dye has a pKa of ~7.5, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF®-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Microplate Reader
Cell Permeability: Cell-Impermeant
Detection Method: Fluorescent
Indicator Properties: Fluorescent Dye-Based
Indicator: pH Indicator
Shipping Condition: Room Temperature
Product Line: SNARF®

Contents & storage

Store at room temperature and protect from light.