Use ProLong® Live Antifade Reagent for brightest fluorescent images with live cells
Invitrogen™
5-(and-6)-Carboxy SNARF™-1
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Description
The cell-impermeant pH indicator, carboxy SNARF®-1 dye has a pKa of ~7.5, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF®-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Indicator Type: | pH Indicator |
|---|---|
| Indicator Properties: | Fluorescent Dye-Based |
| For Use With (Equipment): | Flow Cytometer, Fluorescence Microscope, Microplate Reader |
| Cell Permeability: | Cell-Impermeant |
| Detection Method: | Fluorescent |
| Product Line: | SNARF® |
| Product Size: | 1 mg |
| Shipping Condition: | Room Temperature |
Contents & storage
Store at room temperature and protect from light.
