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5-(and-6)-Carboxy SNARF®-1

Catalog number: C1270

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function

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Description

The cell-impermeant pH indicator, carboxy SNARF®-1 dye has a pKa of ~7.5, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF®-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Indicator Type: pH Indicator
Indicator Properties: Fluorescent Dye-Based
For Use With (Equipment): Flow Cytometer, Fluorescence Microscope, Microplate Reader
Cell Permeability: Cell-Impermeant
Detection Method: Fluorescent
Product Size: 1 mg
Shipping Condition: Room Temperature

Contents & storage

Store at room temperature and protect from light.

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