Calcein: a novel marker for lymphocytes which enter lymph nodes.
AuthorsWeston SA, Parish CR
JournalCytometry
PubMed ID1451604
Previous studies have identified unique cell surface antigens which are associated with the specific binding of lymphocytes to high endothelial venules (HEV). Evidence is presented in this paper which demonstrates that uptake of the fluorescent dye calcein by lymphocytes represents an additional marker for the lymph node homing subpopulation of ... More
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
AuthorsDriessen AJ, van den Hooven HW, Kuiper W, van de Kamp M, Sahl HG, Konings RN, Konings WN
JournalBiochemistry
PubMed ID7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent ... More
Ability of 16 priority PAHs to be photocytotoxic to a cell line from the rainbow trout gill.
'Sixteen polycyclic aromatic hydrocarbons (PAHs) were screened for their ability to be photocytotoxic to a cell line from the rainbow trout gill, RTgill-W1. PAHs could be divided into one of three groups: incapable of being photocytotoxic, able to be both photocytotoxic and directly cytotoxic, or capable of being only photocytotoxic. ... More
Cell death-associated translocation of plasma membrane components induced by CTL.
AuthorsKawasaki Y, Saito T, Shirota-Someya Y, Ikegami Y, Komano H, Lee MH, Froelich CJ, Shinohara N, Takayama H
JournalJ Immunol
PubMed ID10779768
'In the very early stages of target cell apoptosis induced by CTL, we found that fluorescence of labeling probes of the target plasma membrane, such as N-(3-triethylammoniumpropyl)-4-(p-dibutylaminostyryl)pyridin ium dibromide (FM1-43), was translocated into intracellular membrane structures including nuclear envelope and mitochondria. This translocation was associated with the execution of CTL-mediated ... More
Comparison of four different colorimetric and fluorometric cytotoxicity assays in a zebrafish liver cell line.
AuthorsBopp SK, Lettieri T,
JournalBMC Pharmacol
PubMed ID18513395
'BACKGROUND: A broad spectrum of cytotoxicity assays is currently used in the fields of (eco)toxicology and pharmacology. To choose an appropriate assay, different parameters like test compounds, detection mechanism, specificity, and sensitivity have to be considered. Furthermore, tissue or cell line can influence test performance. For zebrafish (Danio rerio), as ... More
An improved double fluorescence flow cytometry method for the quantification of killer cell/target cell conjugate formation.
AuthorsCavarec L, Quillet-Mary A, Fradelizi D, Conjeaud H
JournalJ Immunol Methods
PubMed ID2197333
'We have developed an improved method to analyse stable associations (conjugate formation) between effector and target cells. Hydroethidine (red) stained lymphoblastoid target cells were cocentrifuged with carboxyfluorescein diacetate acetoxymethylester (green) stained human IL-2 activated cytotoxic cells (LAK). In the present studies either enriched or purified CD3 negative large granular lymphocytes ... More
Evaluating the toxicity of Triton X-100 to protozoan, fish, and mammalian cells using fluorescent dyes as indicators of cell viability.
Three viability assays using fluorescent dyes effectively detected a loss of viability in cultures of three mammalian cell lines (H4IIE, Caco2, and HepG-2), two fish cell lines (RTgill-W1 and RTL-W1), and a ciliated protozoan, Tetrahymena thermophila, after exposure to Triton X-100, used as a model toxicant. The dyes were Alamar ... More
Ability of 16 priority PAHs to be directly cytotoxic to a cell line from the rainbow trout gill.
Sixteen polycyclic aromatic hydrocarbons (PAHs) were screened for their ability to be directly cytotoxic to a cell line from the rainbow trout gill, RTgill-W1. Exposure times of 2 h or less were sufficient for direct cytotoxicity to be detected, which appeared to be caused by a common mechanism, the general ... More
Elicitor-activated phospholipase A(2) generates lysophosphatidylcholines that mobilize the vacuolar H(+) pool for pH signaling via the activation of Na(+)-dependent proton fluxes.
AuthorsViehweger K, Dordschbal B, Roos W
JournalPlant Cell
PubMed ID12119371
The elicitation of phytoalexin biosynthesis in cultured cells of California poppy involves a shift of cytoplasmic pH via the transient efflux of vacuolar protons. Intracellular effectors of vacuolar proton transport were identified by a novel in situ approach based on the selective permeabilization of the plasma membrane for molecules of ... More
Fluorescent dyes for lymphocyte migration and proliferation studies.
AuthorsParish CR
JournalImmunol Cell Biol
PubMed ID10571670
Fluorescent dyes are increasingly being exploited to track lymphocyte migration and proliferation. The present paper reviews the properties and performance of some 14 different fluorescent dyes that have been used during the last 20 years to monitor lymphocyte migration. Of the 14 dyes discussed, two stand out as being the ... More
Airborne PAHs inhibit gap junctional intercellular communication and activate MAPKs in human bronchial epithelial cell line.
Authors
JournalEnviron Toxicol Pharmacol
PubMed ID32492535
Repeatability and Reproducibility of the RTgill-W1 Cell Line Assay for Predicting Fish Acute Toxicity.
Authors
JournalToxicol Sci
PubMed ID30825313
Pbx1 is required for adult subventricular zone neurogenesis.
Authors
JournalDevelopment
PubMed ID27226325
Identification of novel drugs to target dormant micrometastases.