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Zitierungen und Referenzen (14)
Invitrogen™
5-CFDA, AM (5-Carboxyfluorescein-Diacetat, Acetoxymethyl-Ester)
5-CFDA, AM ist ein membrangängiges Esterasesubstrat, das als Viabilitätssonde dienen kann, die sowohl die zur Aktivierung seiner Fluoreszenz erforderliche enzymatischeWeitere Informationen
| Katalognummer | Menge |
|---|---|
| C1354 | 5 mg |
Katalognummer C1354
Preis (EUR)
332,65
Exklusiv online
354,00Ersparnis 21,35 (6%)
Each
Menge:
5 mg
Preis (EUR)
332,65
Exklusiv online
354,00Ersparnis 21,35 (6%)
Each
5-CFDA, AM ist ein membrangängiges Esterasesubstrat, das als Viabilitätssonde dienen kann, die sowohl die zur Aktivierung seiner Fluoreszenz erforderliche enzymatische Aktivität als auch die für die intrazelluläre Retention ihres Fluoreszenzprodukts erforderliche Zellmembranintegrität misst. Elektronisch neutrale AM-Ester von CFDA können zur Aufnahme in Zellen in geringeren Konzentrationen als CFDA eingesetzt werden. Nach der Hydrolyse durch intrazelluläre Esterasen liefert dieser AM-Ester auch Carboxyfluorescein: Im Vergleich zu Fluorescein enthält Carboxyfluorescein zusätzliche negative Ladungen und wird daher besser in Zellen aufbewahrt.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
FarbstofftypAndere Etiketten oder Farbstoffe
Menge5 mg
ReagenztypCFDA & zugehörige Verbindungen
VersandbedingungRaumtemperatur, Raumtemperatur
Produkttyp5-CFDA-AM
Unit SizeEach
Inhalt und Lagerung
Im Tiefkühlgerät lagern (-5 bis -30 °C).
Zitierungen und Referenzen (14)
Zitierungen und Referenzen
Abstract
Calcein: a novel marker for lymphocytes which enter lymph nodes.
Journal:Cytometry
PubMed ID:1451604
Previous studies have identified unique cell surface antigens which are associated with the specific binding of lymphocytes to high endothelial venules (HEV). Evidence is presented in this paper which demonstrates that uptake of the fluorescent dye calcein by lymphocytes represents an additional marker for the lymph node homing subpopulation of
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
Journal:Biochemistry
PubMed ID:7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent
Ability of 16 priority PAHs to be photocytotoxic to a cell line from the rainbow trout gill.
Journal:Toxicology
PubMed ID:9699801
'Sixteen polycyclic aromatic hydrocarbons (PAHs) were screened for their ability to be photocytotoxic to a cell line from the rainbow trout gill, RTgill-W1. PAHs could be divided into one of three groups: incapable of being photocytotoxic, able to be both photocytotoxic and directly cytotoxic, or capable of being only photocytotoxic.
Cell death-associated translocation of plasma membrane components induced by CTL.
Journal:J Immunol
PubMed ID:10779768
'In the very early stages of target cell apoptosis induced by CTL, we found that fluorescence of labeling probes of the target plasma membrane, such as N-(3-triethylammoniumpropyl)-4-(p-dibutylaminostyryl)pyridin ium dibromide (FM1-43), was translocated into intracellular membrane structures including nuclear envelope and mitochondria. This translocation was associated with the execution of CTL-mediated
Comparison of four different colorimetric and fluorometric cytotoxicity assays in a zebrafish liver cell line.
Journal:BMC Pharmacol
PubMed ID:18513395
'BACKGROUND: A broad spectrum of cytotoxicity assays is currently used in the fields of (eco)toxicology and pharmacology. To choose an appropriate assay, different parameters like test compounds, detection mechanism, specificity, and sensitivity have to be considered. Furthermore, tissue or cell line can influence test performance. For zebrafish (Danio rerio), as